Si Lingling, Yan Xinyan, Wang Yan, Ren Boxue, Ren Huanhuan, Ding Yangfang, Zheng Qiusheng, Li Defang, Liu Ying
School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai, China.
Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang, China.
Front Oncol. 2020 Apr 2;10:415. doi: 10.3389/fonc.2020.00415. eCollection 2020.
Chamaejasmin B (CHB), a natural biflavone isolated from L., has been reported to exhibit anti-cancer properties; however, its effect in melanoma cells is not clear. Here, we aimed to investigate the anticancer effect of CHB in mouse melanoma B16F0 and B16F10 cells. We found that CHB significantly suppressed cell proliferation and promoted cell cycle arrest at G0/G1 phase in B16F0 cells; it also induced cell differentiation and increased melanin content by increasing tyrosinase (TYR) activity and mRNA levels of melanogenesis-related genes in B16F0 cells. Meanwhile, wound closure, invasion, and migration of B16F0 and B16F10 cells were dramatically inhibited. Moreover, CHB significantly increased ROS levels and decreased ΔΨm, resulting in B16F0 and B16F10 cell apoptosis. Finally, in studies showed that CHB inhibited tumor growth and induced tumor apoptosis in a mouse xenograft model of murine melanoma B16F0 and B16F10 cells. Overall, CHB decreases malignant characteristics and may be a promising therapeutic agent for malignant melanoma cells via multiple signaling pathways.
白花败酱素B（CHB）是一种从[植物名称]中分离出的天然双黄酮，据报道具有抗癌特性；然而，其对黑色素瘤细胞的作用尚不清楚。在此，我们旨在研究CHB对小鼠黑色素瘤B16F0和B16F10细胞的抗癌作用。我们发现，CHB显著抑制B16F0细胞的增殖并促进细胞周期停滞于G0/G1期；它还通过增加B16F0细胞中酪氨酸酶（TYR）活性和黑色素生成相关基因的mRNA水平来诱导细胞分化并增加黑色素含量。同时，B16F0和B16F10细胞的伤口闭合、侵袭和迁移受到显著抑制。此外，CHB显著提高活性氧水平并降低线粒体膜电位（ΔΨm），导致B16F0和B16F10细胞凋亡。最后，体内研究表明，CHB在小鼠黑色素瘤B16F0和B16F10细胞异种移植模型中抑制肿瘤生长并诱导肿瘤凋亡。总体而言，CHB降低恶性特征，可能是通过多种信号通路治疗恶性黑色素瘤细胞的有前景的治疗剂。
