通过平衡分配研究氨酰-tRNA合成酶与转移核糖核酸之间的相互作用。
Studies of the interaction between aminoacyl-tRNA synthetase and transfer ribonucleic acid by equilibrium partition.
作者信息
Hustedt H, Kula M R
出版信息
Eur J Biochem. 1977 Mar 15;74(1):191-8. doi: 10.1111/j.1432-1033.1977.tb11380.x.
The partition behavior of isoleucyl-tRNA synthetase, leucyl-tRNA synthetase and tRNA in aqueous two-phase systems composed of the polymers poly(ethyleneglycol) and dextran was investigated. From the results of this investigation a two-phase system could be derived which can be employed for the study of the interactions between synthetases and their cognate tRNAs by equilibrium partition. These measurements show that in each case one molecule of cognate tRNA is bound per molecule of enzyme. The binding constants were in the range 1-5micronM-1. It could be demonstrated that equilibrium partition is a useful method for the study of interactions between macromolecules.
研究了异亮氨酰 - tRNA合成酶、亮氨酰 - tRNA合成酶和tRNA在由聚乙二醇和葡聚糖聚合物组成的双水相系统中的分配行为。根据该研究结果，可以得到一种双水相系统，该系统可用于通过平衡分配研究合成酶与其同源tRNA之间的相互作用。这些测量结果表明，在每种情况下，每个酶分子结合一个同源tRNA分子。结合常数在1 - 5μM⁻¹范围内。可以证明平衡分配是研究大分子之间相互作用的一种有用方法。
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