血浆和尿液中雷尼替丁的高效液相色谱分析

High-performance liquid chromatographic analysis of ranitidine in plasma and urine.

作者信息

Shiekh Salem M, Gharaibeh A M, Alkaysi H N, Badwan A

机构信息

Faculty of Pharmacy, Jordan University of Science and Technology, Irbid.

出版信息

J Clin Pharm Ther. 1988 Oct;13(5):351-7. doi: 10.1111/j.1365-2710.1988.tb00204.x.

DOI:10.1111/j.1365-2710.1988.tb00204.x

PMID:3230100

Abstract

A high-performance liquid chromatographic method for the analysis of ranitidine in plasma and urine is described. Plasma samples were extracted with dichloromethane while urine samples were injected directly after dilution. The mobile phase consisted of: 0.05 M ammonium acetate buffer containing 0.01 M octane sulphonate, 5.3%; methanol, 31.6%; and acetonitrile, 63.1%. Detection was carried out at 330 nm. Metoclopramide was used as the internal standard. Peak height ratios were measured. Absolute recovery from plasma was 83-85%. Within and between day coefficients of variation ranged from 0.79 to 2.42% and 1.09-2.95% respectively. Plasma and urine samples from a healthy volunteer were analysed.

摘要

描述了一种用于分析血浆和尿液中雷尼替丁的高效液相色谱法。血浆样品用二氯甲烷萃取，而尿液样品在稀释后直接进样。流动相由以下成分组成：含0.01 M辛烷磺酸盐的0.05 M醋酸铵缓冲液，5.3%；甲醇，31.6%；以及乙腈，63.1%。检测在330 nm波长下进行。使用甲氧氯普胺作为内标。测量峰高比。血浆的绝对回收率为83 - 85%。日内和日间变异系数分别为0.79%至2.42%和1.09%至2.95%。分析了一名健康志愿者的血浆和尿液样品。