Segelman A B, Adusumalli V E, Segelman F H
Department of Chemical Biology and Pharmacognosy, College of Pharmacy, Rutgers-The State University, Piscataway, NJ 08854.
J Chromatogr. 1990 Dec 28;535(1-2):287-92. doi: 10.1016/s0021-9673(01)88954-8.
An improved high-performance liquid chromatographic method with UV detection at 313 nm has been developed for quantitation of ranitidine in 100 microliter of rat plasma over the range 25 to 1000 ng/ml. To each sample were added the internal standard (metiamide) and 2 M NaOH. After dichloromethane extraction, the nitrogen-dried extracts were reconstituted in the mobile phase of 0.01 M phosphate buffer-triethylamine-methanol-water (530:5:390:75 v/v). Chromatography on mu Bondapak C18 with quantitation by peak height ratios showed an analyte recovery of 97%; a limit of detection of 10 ng/ml; a precision of 1-10% and an accuracy of 1-5%. About 90 samples can be processed in 24 h.
已开发出一种改进的高效液相色谱法，用于在313nm处进行紫外检测，以定量测定100微升大鼠血浆中浓度范围为25至1000ng/ml的雷尼替丁。向每个样品中加入内标（甲硫咪特）和2M氢氧化钠。经二氯甲烷萃取后，将氮吹干燥的萃取物用0.01M磷酸盐缓冲液 - 三乙胺 - 甲醇 - 水（530:5:390:75 v/v）的流动相复溶。在μ Bondapak C18上进行色谱分析，通过峰高比进行定量，结果表明分析物回收率为97%；检测限为10ng/ml；精密度为1 - 10%，准确度为1 - 5%。24小时内可处理约90个样品。
