Sadik Nermin Abdel Hamid, El-Boghdady Noha Ahmed, Omar Nesreen Nabil, Al-Hamid Hager Abd
Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Biochemistry Department, Faculty of Pharmacy, Modern University for Technology and Information, Cairo, Egypt.
J Food Biochem. 2020 Jul;44(7):e13230. doi: 10.1111/jfbc.13230. Epub 2020 Apr 16.
Cataract is the principal cause of blindness. The enzyme, aldose reductase (AR) is a key player in polyol pathway. Buildup of polyols and oxidative stress are the main causes of cataractogenesis. This study investigated the cytoprotective properties of esculetin and idebenone in galactose-induced cataract. Rats were partitioned into four groups each of ten rats. Control group was fed with normal diet; group 2 rats were fed with galactose diet (50%); groups 3, 4 rats were fed with galactose diet concurrently with either esculetin (50 mg/kg BW) or idebenone (100 mg/kg BW), for 20 days. The study revealed that esculetin and idebenone significantly reduced the elevated levels of Bax/Bcl-2 ratio, malondialdehyde, and DNA fragmentation and increased total antioxidant capacity level in lenses compared to the cataract-induced group. Only esculetin decreased AR, galactitol, and advanced glycated end products levels in lenses. Histopathological examinations supported the biochemical findings. Esculetin and idebenone may have chemopreventive effects for sugar cataract. PRACTICAL APPLICATIONS: Cataract is an age-related disease that might cause blindness in older adult people. Presently, no absolute pharmacological treatment is accessible for cataract. The use of natural products or their derivatives attract particular attention in modern medicines as they are believed to be safer with few or no side effects. Esculetin is a polyphenolic compound found in many medicinal plants. Idebenone is a synthetic analogue of coenzyme Q10. The current study is an approach to explore the anticataract effects of esculetin and idebenone in galactose-induced cataract in rats. Our study proved that both agents have anticataractogenic potentials due to their antioxidant and antiapoptotic properties.
白内障是失明的主要原因。醛糖还原酶（AR）是多元醇途径中的关键酶。多元醇的积累和氧化应激是白内障形成的主要原因。本研究调查了七叶亭和艾地苯醌在半乳糖诱导的白内障中的细胞保护特性。将大鼠分为四组，每组十只。对照组给予正常饮食；第2组大鼠给予半乳糖饮食（50%）；第3、4组大鼠在给予半乳糖饮食的同时分别给予七叶亭（50mg/kg体重）或艾地苯醌（100mg/kg体重），持续20天。研究表明，与白内障诱导组相比，七叶亭和艾地苯醌显著降低了晶状体中Bax/Bcl-2比值、丙二醛和DNA片段化的升高水平，并提高了总抗氧化能力水平。只有七叶亭降低了晶状体中AR、半乳糖醇和晚期糖基化终产物的水平。组织病理学检查支持了生化结果。七叶亭和艾地苯醌可能对糖性白内障具有化学预防作用。实际应用：白内障是一种与年龄相关的疾病，可能导致老年人失明。目前，尚无绝对有效的白内障药物治疗方法。天然产物或其衍生物在现代医学中备受关注，因为它们被认为更安全，副作用很少或没有。七叶亭是一种存在于许多药用植物中的多酚化合物。艾地苯醌是辅酶Q10的合成类似物。本研究旨在探讨七叶亭和艾地苯醌对大鼠半乳糖诱导的白内障中的抗白内障作用。我们的研究证明，由于它们的抗氧化和抗凋亡特性，这两种药物都具有抗白内障的潜力。
