使用手性α1-酸性糖蛋白柱对消旋氨鲁米特及其乙酰化代谢物进行直接液相色谱拆分。
Direct liquid chromatographic resolution of racemic aminoglutethimide and its acetylated metabolite using a chiral alpha 1-acid glycoprotein column.
作者信息
Aboul-Enein H Y, Islam M R
机构信息
Radionuclide and Cyclotron Operations Department, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia.
出版信息
J Chromatogr Sci. 1988 Dec;26(12):616-9. doi: 10.1093/chromsci/26.12.616.
A 10-cm long alpha 1-acid glycoprotein column is used for the enantiomeric resolution of the clinically used racemic aminoglutethimide (+/- AG) and its acetylated metabolite (+/- AAG). A direct liquid chromatographic resolution of racemic aminoglutethimide and its acetylated metabolite is accomplished without any derivatizations. Maximum resolutions of 1.37 and 0.73 are obtained for the enantiomers of aminoglutethimide and its acetylated metabolite, respectively. The effect of the 2-propanol content in mobile phase on retention and enantioselectivity of aminoglutethimide and its acetylated metabolite is demonstrated. The variation of the separation factors (alpha) with pH in enantiomeric separation of aminoglutethimide is also shown.
一根10厘米长的α1-酸性糖蛋白柱用于临床使用的外消旋氨鲁米特（±AG）及其乙酰化代谢物（±AAG）的对映体拆分。无需任何衍生化即可实现外消旋氨鲁米特及其乙酰化代谢物的直接液相色谱拆分。氨鲁米特及其乙酰化代谢物的对映体分别获得了1.37和0.73的最大拆分度。展示了流动相中2-丙醇含量对氨鲁米特及其乙酰化代谢物保留和对映选择性的影响。还显示了氨鲁米特对映体拆分中分离因子（α）随pH的变化。
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