通过对感染的组织培养物进行荧光测定法来早期检测流感病毒。
Early detection of influenza virus by using a fluorometric assay of infected tissue culture.
作者信息
Pachucki C T, Creticos C
机构信息
Veterans Administration Hospital, Hines, Illinois 60141.
出版信息
J Clin Microbiol. 1988 Dec;26(12):2664-6. doi: 10.1128/jcm.26.12.2664-2666.1988.
Abstract
A fluorometric substrate, 4-methylumbelliferyl-alpha-ketoside of N-acetylneuramide, was used directly on clinical specimens and infected tissue culture 24 h after inoculation for the detection of influenza viral neuraminidase. Viral neuraminidase was detected in infected tissue culture but not in clinical specimens. The sensitivity of the assay on tissue culture was 92%, and the specificity was 96%.
摘要
一种荧光底物，N-乙酰神经氨酸的4-甲基伞形酮基-α-酮糖苷，在接种后24小时直接用于临床标本和感染的组织培养物中，以检测流感病毒神经氨酸酶。在感染的组织培养物中检测到了病毒神经氨酸酶，但在临床标本中未检测到。该检测方法对组织培养物的敏感性为92%，特异性为96%。
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