Noyola D E, Paredes A J, Clark B, Demmler G J
Department of Pediatrics, Baylor College of Medicine, Texas Children's Hospital, 6621 Fannin, MC 3-2371, Houston, TX 77030, USA.
Pediatr Dev Pathol. 2000 Mar-Apr;3(2):162-7. doi: 10.1007/s100240050020.
A prototype version of a new diagnostic assay for influenza A and B (Zstat Flutrade mark) based on detection of viral neuraminidase was evaluated and compared to culture in 196 clinical samples. Children with respiratory illnesses were prospectively evaluated at a pediatrician's office and at a large children's hospital using the neuraminidase assay and viral culture performed on respiratory secretions. Influenza virus was isolated from 51 samples and 83 were positive by the neuraminidase assay. When compared to culture the sensitivity of the assay was 96%, specificity was 77%, positive predictive value was 59%, and negative predictive value was 98%. Testing in the laboratory of pure cultures of bacteria and non-influenza viruses frequently found in the respiratory tract showed 0% cross-reactivity with the neuraminidase assay and 100% specificity for influenza virus in vitro. This new assay provided useful information for the preliminary diagnosis of influenza A and B infections and appears to be suitable for both point-of-care use in the physician's office and rapid diagnosis in a virology laboratory. The high sensitivity makes it particularly useful as a screening test for exclusion of influenza A and B infections. To confirm the diagnosis and exclude a false-positive result, as well as to determine the influenza virus type, a viral culture may be considered.
一种基于检测病毒神经氨酸酶的新型甲型和乙型流感诊断检测方法（Zstat Flu商标）的原型版本，在196份临床样本中进行了评估，并与病毒培养法进行了比较。在一家儿科诊所和一家大型儿童医院，对患有呼吸道疾病的儿童进行了前瞻性评估，使用神经氨酸酶检测法和对呼吸道分泌物进行病毒培养。从51份样本中分离出流感病毒，83份样本通过神经氨酸酶检测呈阳性。与病毒培养法相比，该检测方法的敏感性为96%，特异性为77%，阳性预测值为59%，阴性预测值为98%。在实验室对呼吸道中常见的细菌和非流感病毒纯培养物进行检测，结果显示神经氨酸酶检测法的交叉反应率为0%，对流感病毒的体外特异性为100%。这种新的检测方法为甲型和乙型流感感染的初步诊断提供了有用信息，似乎既适用于医生办公室的即时检测，也适用于病毒学实验室的快速诊断。高敏感性使其作为排除甲型和乙型流感感染的筛查试验特别有用。为了确诊并排除假阳性结果，以及确定流感病毒类型，可考虑进行病毒培养。
