Laboratory of Veterinary Pathology, Co-department of Veterinary Medicine, Iwate University, Morioka, Japan.
Gifu Prefectural Meat Inspection Office, Ogaki, Japan.
Avian Pathol. 2020 Oct;49(5):440-447. doi: 10.1080/03079457.2020.1757621. Epub 2020 May 18.
The prototype fowl glioma-inducing virus (FGVp) causes fowl glioma and cerebellar hypoplasia in chickens. In this study, we investigated whether a strain of avian leukosis virus (ALV), associated with avian osteopetrosis and mesenchymal neoplasms, is able to induce fowl glioma. We encountered avian osteopetrosis and mesenchymal neoplasms, including myxosarcoma and rhabdomyosarcoma, in Japanese native chickens used for both egg-laying and meat production. These birds were also affected by non-suppurative encephalitis and glioma in their brains. Four ALV strains (GifN_001, GifN_002, GifN_004, GifN_005) were isolated, and a phylogenic analysis of SU showed that these isolates were classified into different clusters from FGVp and the variants previously reported. Whereas the SU shared a high identity (94.7%) with that of Rous sarcoma virus (strain Schmidt-Ruppin B) (RSV-SRB), the identity between TM of GifN_001 and that of FGVp was high (94.5%), indicating that GifN_strains may emerge by recombination between FGVp and other exogenous ALVs. Specific-pathogen-free chickens inoculated with GifN_001 revealed fowl glioma and cerebellar hypoplasia. These results suggest that the newly isolated strains have acquired neuropathogenicity to chickens.
雏鸡致神经胶质瘤病毒（FGVp）原型可引起鸡神经胶质瘤和小脑发育不全。本研究旨在探讨与禽骨髓性骨石化和间叶性肿瘤相关的禽白血病病毒（ALV）是否能诱发鸡神经胶质瘤。我们在用于产蛋和产肉的日本本地鸡中发现了禽骨髓性骨石化和间叶性肿瘤，包括粘液肉瘤和横纹肌肉瘤，这些鸟类还患有非化脓性脑炎和脑部神经胶质瘤。我们分离到了 4 株 ALV（GifN_001、GifN_002、GifN_004、GifN_005），SU 的系统发育分析表明，这些分离株与 FGVp 和以前报道的变异株属于不同的聚类。尽管 SU 与劳斯肉瘤病毒（Schmidt-Ruppin B 株）（RSV-SRB）的相似度很高（94.7%），但 GifN_001 的 TM 与 FGVp 的相似度很高（94.5%），表明 GifN_株可能是由 FGVp 与其他外源性 ALV 重组产生的。用 GifN_001 接种 SPF 鸡会导致鸡神经胶质瘤和小脑发育不全。这些结果表明，新分离的毒株已获得对鸡的神经致病性。
