Liu Gong-Zhen, Zhang Hong-Hai, Liu Qing, Qiu Bo, Wang Feng, Wang Xiao-Wei, Chen Hong-Bo, Cheng Zi-Qiang
College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.
Bing Du Xue Bao. 2009 Nov;25(6):445-51.
Two strains of Avian leukosis virus subgroup B (ALV-B) were isolated for the first time in China Hy-line White on the cultured DF-1 cells which were inoculated tissue samples from by an ELISA assay, a histopathology examination and a PCR-based diagnosis. The results from the ELISA assay indicated that the positive rate of serum antibodies to ALV-B and ALV-J virus were 16.3% (15/92) and 13% (12/92), respectively. The histopathological examination indicated that two types of tumor cells existed at same focus in liver and spleen, which mainly were myelocytoma cells and lymphosarcoma cells. The PCR-based diagnosis were performed as follows: the cellular DNA was extracted from the inoculated DF-1 cells; the specific fragments of 1100 bp and 924 bp were obtained by a PCR system with the diagnostic primers of ALV-B and ALV-J; and the PCR results for ALV-A, MDV and REV were all negative. Then, the amplified fragments of the two ALV-B stains were partially sequenced and shown an identity of 92.8%,94.7% with the prototype strain of ALV-B (RSV Schmidt-ruppin B). The identities of two ALV-J strains with the prototype strain HPRS-103 at 96.9%, 91.5%; The identities of two ALV-J strains with the American prototype strain at 85.9%, 81.5%. Our study had shown that ALV-B was isolated for the first time from the ALV-J infected commercial layer flocks in China. It also indicated that the chance of genetic recombination among various subgroups of ALV was increased.
在中国海兰白鸡中首次分离出两株B亚群禽白血病病毒（ALV-B），采用酶联免疫吸附测定（ELISA）、组织病理学检查和基于聚合酶链反应（PCR）的诊断方法，从接种的组织样本中在DF-1细胞上进行培养。ELISA检测结果表明，血清中抗ALV-B和ALV-J病毒抗体的阳性率分别为16.3%（15/92）和13%（12/92）。组织病理学检查表明，肝脏和脾脏的同一病灶中存在两种类型的肿瘤细胞，主要是骨髓细胞瘤细胞和淋巴肉瘤细胞。基于PCR的诊断如下：从接种的DF-1细胞中提取细胞DNA；使用ALV-B和ALV-J的诊断引物通过PCR系统获得1100 bp和924 bp的特异性片段；ALV-A、马立克氏病病毒（MDV）和网状内皮组织增殖症病毒（REV）的PCR结果均为阴性。然后，对两株ALV-B毒株的扩增片段进行部分测序，与ALV-B原型毒株（劳斯肉瘤病毒施密特-鲁平B株，RSV Schmidt-ruppin B）的同源性分别为92.8%、94.7%；两株ALV-J毒株与原型毒株HPRS-103的同源性分别为96.9%、91.5%；两株ALV-J毒株与美国原型毒株的同源性分别为85.9%、81.5%。我们的研究表明，在中国首次从感染ALV-J的商品蛋鸡群中分离出ALV-B。这也表明ALV各亚群之间发生基因重组的可能性增加。
