Department of Biomaterials, College of Materials, Xiamen University, Xiamen, Fujian 361005, China.
Analyst. 2020 Jun 7;145(11):3916-3921. doi: 10.1039/d0an00451k. Epub 2020 Apr 17.
Biothiols play an important role in living cells and are associated with many diseases. Thus, it is necessary to develop a facile, cost-effective, and convenient analytical method for the detection of biothiols. Nanozymes are functional nanomaterials with enzymatic activities. Due to their unique advantages (e.g., low cost, high stability, and multifunctionality), nanozymes have been extensively used to construct sensing systems. Previous studies demonstrated colorimetric assays for biothiol detection because they could competitively inhibit the peroxidase-like activities of nanozymes. However, few studies were able to differentiate biothiols from each other. To address these challenges, herein, we first synthesized Au alloy nanozymes with better peroxidase-like activities than gold nanoparticles (AuNPs). Then, cross-reactive sensor arrays were constructed with three alloy nanozymes. Six typical biothiols (i.e., glutathione, cysteine, dithiothreitol, mercaptoacetic acid, mercaptoethanol, and mercaptosuccinic acid) were successfully detected and discriminated by the as-prepared nanozyme sensor arrays. Moreover, the practical application of the nanozyme sensor arrays was demonstrated by discriminating biothiols in serum successfully.
生物硫醇在活细胞中起着重要作用，与许多疾病有关。因此，有必要开发一种简便、经济高效且方便的分析方法来检测生物硫醇。纳米酶是具有酶活性的功能纳米材料。由于其独特的优势（例如，低成本、高稳定性和多功能性），纳米酶已被广泛用于构建传感系统。先前的研究表明，比色法测定生物硫醇的检测，因为它们可以竞争性地抑制纳米酶的过氧化物酶样活性。然而，很少有研究能够区分彼此的生物硫醇。为了解决这些挑战，本文首次合成了具有比金纳米粒子（AuNPs）更好的过氧化物酶样活性的 Au 合金纳米酶。然后，使用三种合金纳米酶构建了交叉反应传感器阵列。通过所制备的纳米酶传感器阵列成功检测和区分了六种典型的生物硫醇（即谷胱甘肽、半胱氨酸、二硫苏糖醇、巯基乙酸、巯基乙醇和巯基丁二酸）。此外，通过成功区分血清中的生物硫醇，证明了纳米酶传感器阵列的实际应用。
