Department of Chemistry, Key Laboratory of Biomedical Functional Materials, School of Science, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210000, People's Republic of China.
ACS Appl Mater Interfaces. 2024 Jul 10;16(27):34538-34548. doi: 10.1021/acsami.4c04844. Epub 2024 Jun 28.
Biothiol assays offer vital insights into health assessment and facilitate the early detection of potential health issues, thereby enabling timely and effective interventions. In this study, we developed ultrasmall CuMn-Histidine (His) nanozymes with multiple enzymatic activities. CuMn-His enhanced peroxidase (POD)-like activity at neutral pH was achieved through hydrogen bonding and electrostatic effects. In addition, CuMn-His possesses laccase (LAC)-like and superoxide dismutase (SOD)-like activities at neutral pH. Based on three different enzyme mimetic activities of CuMn-His at neutral pH, the colorimetric sensing array without changing the buffer solution was successfully constructed. The array was successfully used for the identification of three biothiols, glutathione (GSH), cysteine (Cys), and homocysteine (Hcy). Subsequently, excellent application results were shown in complex serum and cellular level analyses. This study provides an innovative strategy for the development of ultrasmall bimetallic nanozymes with multiple enzymatic activities and the construction of colorimetric sensing arrays.
生物硫醇分析为健康评估提供了重要的见解，并有助于早期发现潜在的健康问题，从而实现及时有效的干预。在这项研究中，我们开发了具有多种酶活性的超小 CuMn-组氨酸 (His) 纳米酶。通过氢键和静电作用，CuMn-His 在中性 pH 下增强了过氧化物酶 (POD) 样活性。此外，CuMn-His 在中性 pH 下还具有漆酶 (LAC) 样和超氧化物歧化酶 (SOD) 样活性。基于 CuMn-His 在中性 pH 下的三种不同酶模拟活性，成功构建了无需改变缓冲溶液的比色传感阵列。该阵列成功用于三种生物硫醇（谷胱甘肽 (GSH)、半胱氨酸 (Cys) 和同型半胱氨酸 (Hcy)）的识别。随后，在复杂的血清和细胞水平分析中显示出了优异的应用结果。本研究为开发具有多种酶活性的超小双金属纳米酶和构建比色传感阵列提供了一种创新策略。
