Molecular Oncology Lab, Department of Biochemistry, University of Madras, Chennai, India.
Environ Toxicol. 2020 Sep;35(9):971-981. doi: 10.1002/tox.22933. Epub 2020 Apr 17.
Hepatocellular carcinoma (HCC) ranks the sixth position among various cancers worldwide. Recent research shows that natural and dietary compounds possess many therapeutic effects. Citral is a monoterpene aldehyde that contains geranial and neral. The present study was considered to study the role of citral against N-nitrosodiethylamine (NDEA)-induced HCC via modulation of antioxidants and xenobiotic-metabolizing enzymes in vivo. NDEA-alone-administered group II animals profoundly showed increased tumor incidence, reactive oxygen species, liver marker enzyme levels, serum bilirubin levels, tumor markers of carcinoembryonic antigen, α-fetoprotein, proliferative markers of argyrophilic nucleolar organizing regions, proliferating cell nuclear antigen (PCNA) expressions, phase I xenobiotic-metabolic enzymes and simultaneously decreased antioxidants, and phase II enzymes levels. Citral (100 mg/kg b.w.) treatment significantly reverted the levels in group III cancer-bearing animals when compared to group II cancer-bearing animals. In group IV animals, citral-alone administration did not produce any adverse effect during the experimental condition. Based on the results, citral significantly inhibits the hepatocellular carcinogenesis through restoring the antioxidants and phase II xenobiotic-enzyme levels; thereby, it strongly proves as an antiproliferative agent against rat HCC.
肝细胞癌 (HCC) 在全球各种癌症中排名第六。最近的研究表明，天然和饮食化合物具有许多治疗作用。柠檬醛是一种含有橙花醛和柠檬醛的单萜醛。本研究旨在通过体内调节抗氧化剂和异生物质代谢酶来研究柠檬醛对 N-亚硝二乙胺 (NDEA) 诱导的 HCC 的作用。仅给予 NDEA 的第 II 组动物表现出肿瘤发生率、活性氧、肝标记酶水平、血清胆红素水平、癌胚抗原、甲胎蛋白等肿瘤标志物、嗜银核仁组成区的增殖标记物、增殖细胞核抗原 (PCNA) 表达、I 相异生物质代谢酶的显著增加，同时降低抗氧化剂和 II 相酶的水平。与第 II 组荷瘤动物相比，柠檬醛 (100mg/kg b.w.) 治疗可显著逆转第 III 组荷瘤动物的水平。在第 IV 组动物中，柠檬醛单独给药在实验条件下没有产生任何不良反应。基于这些结果，柠檬醛通过恢复抗氧化剂和 II 相异生物质酶水平显著抑制肝细胞癌的发生；因此，它强烈证明是一种抗大鼠 HCC 的增殖剂。
