Departamento de Bioinformática y Biotecnología Genómica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México.
Laboratorio de Investigación en Enfermedades Infecciosas; Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, México City, Edo. de México, México.
PLoS One. 2020 Apr 17;15(4):e0231829. doi: 10.1371/journal.pone.0231829. eCollection 2020.
Acinetobacter baumannii is an opportunistic infectious agent that affects primarily immunocompromised individuals. A. baumannii is highly prevalent in hospital settings being commonly associated with nosocomial transmission and drug resistance. Here, we report the identification and genetic characterization of A. baumannii strains among patients in a tertiary level hospital in Mexico. Whole genome sequencing analysis was performed to establish their genetic relationship and drug resistance mutations profile. Ten genetically different, extensively drug resistant strains were identified circulating among seven wards. The genetic profiles showed resistance primarily against aminoglycosides and beta-lactam antibiotics. Importantly, no mutants conferring resistance to colistin were observed. The results highlight the importance of implementing robust classification schemes for advanced genetic characterization of A. baumannii clinical isolates and simultaneous detection of drug resistance markers for adequate patient's management in clinical settings.
鲍曼不动杆菌是一种机会性感染病原体，主要影响免疫功能低下的个体。鲍曼不动杆菌在医院环境中高度流行，通常与医院内传播和耐药性有关。在这里，我们报告了在墨西哥一家三级医院的患者中鲍曼不动杆菌菌株的鉴定和遗传特征。进行了全基因组测序分析，以确定它们的遗传关系和耐药突变谱。在七个病房中发现了十种遗传上不同的、广泛耐药的菌株。遗传谱显示主要对氨基糖苷类和β-内酰胺类抗生素耐药。重要的是，没有观察到对粘菌素耐药的突变体。这些结果强调了在临床环境中对鲍曼不动杆菌临床分离株进行高级遗传特征分析和同时检测耐药性标志物以进行适当患者管理的重要性。
