Department of Clinical Laboratory, Shunde Hospital, Southern Medical University (the First People's Hospital of Shunde), Foshan 528000, China.
National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Veterinary Drugs Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China.
J Glob Antimicrob Resist. 2019 Mar;16:1-3. doi: 10.1016/j.jgar.2018.11.008. Epub 2018 Nov 13.
The rapid spread of multidrug-resistant (MDR) Acinetobacter baumannii poses a substantial threat for morbidity and mortality worldwide. In particular, carbapenem-resistant A. baumannii has caused a severe challenge to human health. Here we reported the draft genome sequence of A. baumannii S131434, an OXA-23, OXA-66, ADC-25 and TEM-1D co-producing strain recovered from a patient with neonatal pneumonia in China and belonging to the globally disseminated sequence type 195 (ST195) of clonal complex 92 (CC92).
Genomic DNA was sequenced using an Illumina HiSeq platform and the reads were de novo assembled into contigs using CLC Genomics Workbench. The assembled contigs were annotated and bioinformatics analysis was performed.
The genome comprised a circular chromosome of 3898344bp. The presence of the bla, bla, bla and bla genes was detected. In addition, genes conferring resistance to aminoglycosides, macrolides and tetracycline were also identified. Antimicrobial susceptibility testing revealed that the isolate was resistant to all of the tested antibiotics except for polymyxin B, piperacillin/sulbactam and trimethoprim/sulfamethoxazole.
To our knowledge, this is the first report of a clinical A. baumannii ST195 (CC92) isolate producing OXA-23, OXA-66, ADC-25 and TEM-1D in southern China. This draft genome will facilitate further our understanding of the antimicrobial resistance and pathogenic mechanisms in this strain and provides valuable information regarding the colonisation and adaptation of MDR pathogens.
多重耐药（MDR）鲍曼不动杆菌的快速传播对全球的发病率和死亡率构成了重大威胁。特别是耐碳青霉烯类鲍曼不动杆菌对人类健康造成了严重挑战。本研究报道了一株产 OXA-23、OXA-66、ADC-25 和 TEM-1D 的鲍曼不动杆菌 S131434 的基因组序列，该菌株从一名中国新生儿肺炎患者中分离得到，属于全球流行的克隆复合体 92（CC92）的 195 型（ST195）序列型。
使用 Illumina HiSeq 平台对基因组 DNA 进行测序，使用 CLC Genomics Workbench 将读取的序列从头组装成 contigs。对组装的 contigs 进行注释和生物信息学分析。
基因组由一个 3898344bp 的环状染色体组成。检测到 bla、bla、bla 和 bla 基因的存在。此外，还鉴定出了赋予对氨基糖苷类、大环内酯类和四环素类药物耐药的基因。药敏试验结果显示，该分离株除对多黏菌素 B、哌拉西林/他唑巴坦和复方磺胺甲噁唑外，对所有测试的抗生素均耐药。
据我们所知，这是首次报道中国南方地区产 OXA-23、OXA-66、ADC-25 和 TEM-1D 的临床鲍曼不动杆菌 ST195（CC92）分离株。该基因组草图将有助于我们进一步了解该菌株的抗菌药物耐药性和致病机制，并为了解 MDR 病原体的定植和适应提供有价值的信息。
