Biomedical Engineering, Columbia University Fu Foundation School of Engineering and Applied Science, New York, NY, USA.
Department of Radiology and Biomedical Imaging, Yale University, New Haven, CT, USA.
Anal Biochem. 2020 Jun 15;599:113738. doi: 10.1016/j.ab.2020.113738. Epub 2020 Apr 14.
Typical magnetic resonance spectroscopy J-editing methods designed to quantify GABA suffer from contamination of both overlapping macromolecules and homocarnosine signal, introducing potential confounds. The aim of this study was to develop a novel method to assess accurately both the relative concentrations of homocarnosine as well as GABA free from overlapping creatine, homocarnosine and macromolecule signal. A novel method which utilized the combination of echo time STEAM and MEGA-sLASER magnetic resonance spectroscopy experiments at 7T were used to quantify the concentration of GABA and homocarnsoine independently, which are typically quantified in tandem. The metabolites GABA and homocarnosine were measured in brain of 6 healthy control subjects, and in a single subject medicated with isoniazid. It was found that (16.6±10.2)% of the supposed GABA signal in the brain originated from homocarnosine, and that isoniazid caused significantly elevated concentration of GABA and homocarnosine in a single subject compared to controls.
典型的用于定量 GABA 的磁共振波谱 J 编辑方法受到重叠的大分子和同型瓜氨酸信号的污染，引入了潜在的混淆因素。本研究的目的是开发一种新方法，以准确评估同型瓜氨酸以及 GABA 的相对浓度，而不受重叠的肌酸、同型瓜氨酸和大分子信号的干扰。该方法利用了在 7T 下的回波时间 STEAM 和 MEGA-sLASER 磁共振波谱实验的组合，用于独立定量 GABA 和同型瓜氨酸的浓度，这两种浓度通常是串联定量的。该代谢物 GABA 和同型瓜氨酸在 6 名健康对照者的大脑中以及在一名服用异烟肼的单一受试者中进行了测量。结果发现，（16.6±10.2）%的大脑中所谓的 GABA 信号来源于同型瓜氨酸，并且与对照组相比，异烟肼导致单一受试者中 GABA 和同型瓜氨酸的浓度显著升高。
