Natural Resources Program, Center for Natural Resources Studies (CERNA), Mato Grosso do Sul State University (UEMS), Dourados, Mato Grosso do Sul, 79804-970, Brazil.
Applied Optics Group, Faculty of Science and Technology, Federal University of Grande Dourados (UFGD), Dourados, Mato Grosso do Sul, 79804-970, Brazil.
J Hazard Mater. 2020 Sep 5;396:122484. doi: 10.1016/j.jhazmat.2020.122484. Epub 2020 Mar 6.
This work has assessed the impact of copper oxide nanoparticles (CuONPs), designed via green route, toward photosynthetic apparatus on aquatic photoautotrophic organisms. In order to filling knowledge gaps, in vitro and in vivo assays were performed, using cyanobacterial phycocyanin (C-PC) from Arthrospira platensis and Lemna valdiviana plants (duckweed), respectively. Impairment in light energy transfer became evident in C-PC exposed to CuONPs, giving rise to an increase of light absorption and a suppression of fluorescence emission. Fourier transform infrared spectroscopy (FTIR) results showed that C-PC structures might be altered by the nanoparticles, also revealed that CuONPs preferably interacts with -NH functional groups. The data also revealed that CuONPs affected the chlorophyll a content in duckweed leaves. In addition, photosystem II (PSII) performance was significantly affected by CuONPs, negatively impacting the PSII photochemical network. In summary, the results point out that, even eco-friendly designed, CuONPs may negatively affect the photosynthetic process when accumulated by aquatic photoautotrophs.
本研究评估了通过绿色途径设计的氧化铜纳米颗粒（CuONPs）对水生光合生物光合作用装置的影响。为了填补知识空白，分别使用来自节旋藻的藻蓝蛋白（C-PC）和浮萍植物（浮萍）进行了体外和体内测定。暴露于 CuONPs 的 C-PC 中明显出现了光能传递的损伤，导致光吸收增加和荧光发射抑制。傅里叶变换红外光谱（FTIR）结果表明，纳米颗粒可能改变了 C-PC 的结构，还表明 CuONPs 优先与-NH 官能团相互作用。这些数据还表明，CuONPs 影响了浮萍叶片中的叶绿素 a 含量。此外，CuONPs 显著影响 PSII 性能，对 PSII 光化学网络产生负面影响。总之，研究结果表明，即使是环保设计的 CuONPs，当被水生光合自养生物积累时，也可能对光合作用过程产生负面影响。
