Quadros Maurício de, Bugs Roberto Carlos Freitas, Soares Renata de Oliveira, Rossato Adriana Medianeira, Rocha Lisiane da Luz, d'Azevedo Pedro Alves
Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil; Department of Dermatology, Hospital Santa Casa de Misericórdia de Porto Alegre, Porto Alegre, RS, Brazil.
Gram-positive Cocci Laboratory, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, RS, Brazil.
An Bras Dermatol. 2020 May-Jun;95(3):298-306. doi: 10.1016/j.abd.2019.11.004. Epub 2020 Mar 21.
The increasingly frequent use of dermoscopy makes us think about the possibility of transfer of microorganisms, through the dermatoscope, between doctor and patients.
To identify the most frequent gram-positive cocci in dermatoscopes and smartphone adapters, as well as the resistance profile, and to evaluate the factors associated with a higher risk of bacterial contamination of the dermatoscopes.
A cross-sectional study was carried out with 118 dermatologists from Porto Alegre/Brazil between September 2017 and July 2018. Gram-positive cocci were identified by MALDI-TOF MS and habits of use of the dermatoscope were evaluated through an anonymous questionnaire.
Of the dermatoscopes analysed, 46.6% had growth of gram-positive cocci on the lens and 37.3% on the on/off button. The microorganisms most frequently found were S. epidermidis, S. hominis and S. warneri. Attending a hospital, using the dermatoscope at the hospital, with inpatients and in the intensive care unit were significantly associated with colonisation by gram-positive cocci. The highest resistance rates were observed for penicillin, erythromycin and sulfamethoxazole-trimethoprim.
The non-search of gram-negative bacilli, fungi and viruses. Moreover, the small number of adapters did not make it possible to better define if the frequency differences were statistically significant.
Coagulase-negative staphylococci were frequently identified. S. aureus was detected only on the lens.
皮肤镜的使用日益频繁，这使我们思考微生物通过皮肤镜在医生和患者之间传播的可能性。
确定皮肤镜和智能手机适配器中最常见的革兰氏阳性球菌及其耐药情况，并评估与皮肤镜细菌污染风险较高相关的因素。
2017年9月至2018年7月，对来自巴西阿雷格里港的118名皮肤科医生进行了一项横断面研究。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定革兰氏阳性球菌，并通过匿名问卷评估皮肤镜的使用习惯。
在分析的皮肤镜中，46.6%的镜片上有革兰氏阳性球菌生长，37.3%的开/关按钮上有。最常发现的微生物是表皮葡萄球菌、人葡萄球菌和沃氏葡萄球菌。在医院就诊、在医院使用皮肤镜、用于住院患者和重症监护病房与革兰氏阳性球菌定植显著相关。青霉素、红霉素和磺胺甲恶唑-甲氧苄啶的耐药率最高。
未检测革兰氏阴性杆菌、真菌和病毒。此外，适配器数量较少，无法更好地确定频率差异是否具有统计学意义。
经常鉴定出凝固酶阴性葡萄球菌。仅在镜片上检测到金黄色葡萄球菌。
