Department of Viroscience, Erasmus University Medical Center, Rotterdam, Netherlands.
Viroclinics Xplore, Schaijk, Netherlands.
Science. 2020 May 29;368(6494):1012-1015. doi: 10.1126/science.abb7314. Epub 2020 Apr 17.
The current pandemic coronavirus, severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), was recently identified in patients with an acute respiratory syndrome, coronavirus disease 2019 (COVID-19). To compare its pathogenesis with that of previously emerging coronaviruses, we inoculated cynomolgus macaques with SARS-CoV-2 or Middle East respiratory syndrome (MERS)-CoV and compared the pathology and virology with historical reports of SARS-CoV infections. In SARS-CoV-2-infected macaques, virus was excreted from nose and throat in the absence of clinical signs and detected in type I and II pneumocytes in foci of diffuse alveolar damage and in ciliated epithelial cells of nasal, bronchial, and bronchiolar mucosae. In SARS-CoV infection, lung lesions were typically more severe, whereas they were milder in MERS-CoV infection, where virus was detected mainly in type II pneumocytes. These data show that SARS-CoV-2 causes COVID-19-like disease in macaques and provides a new model to test preventive and therapeutic strategies.
目前的大流行冠状病毒，即严重急性呼吸综合征冠状病毒 2（SARS-CoV-2），最近在患有急性呼吸综合征的患者中被发现，即 2019 年冠状病毒病（COVID-19）。为了比较其发病机制与以前出现的冠状病毒的发病机制，我们用 SARS-CoV-2 或中东呼吸综合征（MERS）-CoV 接种食蟹猴，并将其病理学和病毒学与 SARS-CoV 感染的历史报告进行了比较。在感染 SARS-CoV-2 的猕猴中，病毒在没有临床症状的情况下从鼻子和喉咙排出，并在弥漫性肺泡损伤的灶中和 I 型和 II 型肺泡细胞中以及鼻、支气管和细支气管黏膜的纤毛上皮细胞中检测到。在 SARS-CoV 感染中，肺部病变通常更严重，而在 MERS-CoV 感染中则较轻，在 MERS-CoV 感染中，病毒主要在 II 型肺泡细胞中检测到。这些数据表明，SARS-CoV-2 可在猕猴中引起 COVID-19 样疾病，并为测试预防和治疗策略提供了新模型。
