State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, 150090, China.
College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, 056038, China.
Bioprocess Biosyst Eng. 2020 Aug;43(8):1521-1531. doi: 10.1007/s00449-020-02345-1. Epub 2020 Apr 18.
Sulfamethazine (SM2) is one of the sulfonamide antibiotics that is frequently detected in aquatic environment. Given the complex structure of SM2 and its potential threat to the environment, it is necessary to determine the degradation behavior of high-concentration SM2. The mechanisms of community structure and diversity of activated sludge were analyzed. A novel SM2-degrading strain YL1 was isolated which can degrade SM2 with high concentration of 100 mg L. Strain YL1 was identified as Paenarthrobacter ureafaciens and there was also a significant increase in the genus during acclimation. Additional SM2 metabolic mechanisms and genomic information of YL1 were analyzed for further research. The succession of the community structure also investigated the effect of SM2 on the activated sludge. This result not only advances the current understanding of microbial ecology in activated sludge, but also has practical implications for the design and operation of the environmental bioprocesses for treatment of antimicrobial-bearing waste streams.
磺胺甲噁唑（SM2）是一种经常在水环境中检测到的磺胺类抗生素。鉴于 SM2 的复杂结构及其对环境的潜在威胁，有必要确定高浓度 SM2 的降解行为。分析了活性污泥的群落结构和多样性机制。分离到一株能够以 100mg/L 的高浓度降解 SM2 的新型 SM2 降解菌 YL1。菌株 YL1 被鉴定为解脲节杆菌，在驯化过程中该属的数量也显著增加。进一步分析了 YL1 的 SM2 代谢机制和基因组信息，以进行进一步的研究。还研究了群落结构的演替，以考察 SM2 对活性污泥的影响。这一结果不仅推进了人们对活性污泥中微生物生态学的现有认识，而且对含抗菌剂废水的环境生物处理工艺的设计和运行具有实际意义。
