微小 RNA-455-5p 通过靶向 S1PR1 并阻断 mTOR 通路对宫颈癌发挥抑制作用。
MicroRNA-455-5p exerts inhibitory effect in cervical carcinoma through targeting S1PR1 and blocking mTOR pathway.
机构信息
Department of Gynaecology and Obstetrics, DongDa Hospital of Shanxian, Shanxian, Shandong, People's Republic of China.
Medical Clinic, Yuhuangding Hospital of Yantai, Yantai, Shandong, People's Republic of China.
出版信息
Arch Gynecol Obstet. 2020 May;301(5):1307-1315. doi: 10.1007/s00404-020-05536-z. Epub 2020 Apr 17.
BACKGROUND
MicroRNAs (miRNAs) have been increasingly exploited in human malignancies. The regulation of microRNA-455-5p (miR-455-5p) has been shown in several cancers, except for cervical carcinoma. Therefore, the role of miR-455-5p was exploited in cervical carcinoma.
METHODS
The qRT-PCR experiment was used to assess miR-455-5p and S1PR1 expression levels. We explored the function of miR-455-5p through MTT and Transwell assays. The mTOR pathway and cell apoptosis were detected by Western blot assays. The relationship between miR-455-5p and S1PR1 was testified by dual-luciferase reporter assay.
RESULTS
MiR-455-5p expression was decreased in cervical carcinoma, which was related to poor clinical outcome in cervical carcinoma patients. MiR-455-5p inhibited cell viability and metastasis in cervical carcinoma. Further, S1PR1 is a direct target of miR-455-5p. S1PR1 recovered the inhibition of cell viability and metastasis induced by miR-455-5p in cervical carcinoma. In addition, miR-455-5p induced cell apoptosis and inactivated the mTOR pathway in cervical carcinoma.
CONCLUSION
MiR-455-5p exerts inhibitory effect in cervical carcinoma through targeting S1PR1 and blocking the mTOR pathway.
背景
MicroRNAs (miRNAs) 在人类恶性肿瘤中得到了越来越多的应用。miR-455-5p 的调控在几种癌症中得到了证实，除了宫颈癌。因此，我们研究了 miR-455-5p 在宫颈癌中的作用。
方法
采用 qRT-PCR 实验检测 miR-455-5p 和 S1PR1 的表达水平。通过 MTT 和 Transwell 实验探索 miR-455-5p 的功能。通过 Western blot 实验检测 mTOR 通路和细胞凋亡。通过双荧光素酶报告实验验证 miR-455-5p 和 S1PR1 之间的关系。
结果
miR-455-5p 在宫颈癌中表达降低，与宫颈癌患者的不良临床结局相关。miR-455-5p 抑制宫颈癌细胞的活力和转移。进一步研究表明，S1PR1 是 miR-455-5p 的直接靶基因。S1PR1 恢复了 miR-455-5p 对宫颈癌细胞活力和转移的抑制作用。此外，miR-455-5p 诱导宫颈癌细胞凋亡并抑制 mTOR 通路。
结论
miR-455-5p 通过靶向 S1PR1 并阻断 mTOR 通路在宫颈癌中发挥抑制作用。
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