不同种类的 extender 对大熊猫（Ailuropoda melanoleuca）冻精解冻后品质的影响。
Effects of extender type on the quality of post-thaw giant panda (Ailuropoda melanoleuca) semen.
机构信息
Chengdu Research Base of Giant Panda Breeding, 610000, Chengdu, Sichuan Province, China; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, 610000, Chengdu, Sichuan Province, China; Sichuan Academy of Giant Panda, 610000, Chengdu, Sichuan Province, China.
Chengdu Research Base of Giant Panda Breeding, 610000, Chengdu, Sichuan Province, China; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, 610000, Chengdu, Sichuan Province, China; Sichuan Academy of Giant Panda, 610000, Chengdu, Sichuan Province, China.
出版信息
Cryobiology. 2020 Jun;94:95-99. doi: 10.1016/j.cryobiol.2020.04.003. Epub 2020 Apr 15.
Sperm cryopreservation is an essential approach for assisted reproduction and genetic resources conservation in captive giant pandas. Cryopreservation, however, leads to a significant decrease in sperm quality and, consequently, a low fertilization rate. Therefore, it is mandatory to disclose more suitable and efficient freezing strategies for sperm cryopreservation. In the present study, we compared for the first time the performance of two commercial freeze extender (INRA96 versus TEST) freezing methods on post-thawed semen quality. Semen cryopreserved with the INRA96 showed better total motility (73.00 ± 4.84% vs 57.56 ± 3.60%, P < 0.001), membrane integrity (60.92 ± 2.27% vs 40.53 ± 2.97%, P < 0.001) and acrosome integrity (90.39 ± 2.74% vs 84.26 ± 4.27%, P < 0.05) than stored with TEST. There was no significant difference in DNA integrity after thawing between the two extenders (95.69 ± 3.60% vs 94.26 ± 4.84%). In conclusion, the INRA96 method showed to be better for giant panda sperm cryopreservation and should therefore be recommended for use in order to increase success of artificial insemination.
精子冷冻保存是辅助生殖和圈养大熊猫遗传资源保护的重要方法。然而，冷冻保存会导致精子质量显著下降，从而受精率降低。因此，必须揭示更合适和有效的精子冷冻保存的冷冻策略。在本研究中，我们首次比较了两种商业冷冻液（INRA96 与 TEST）在解冻后精子质量上的性能。用 INRA96 冷冻的精子总活力（73.00±4.84%比 57.56±3.60%，P<0.001）、膜完整性（60.92±2.27%比 40.53±2.97%，P<0.001）和顶体完整性（90.39±2.74%比 84.26±4.27%，P<0.05）均优于用 TEST 冷冻的精子。两种冷冻液解冻后 DNA 完整性无显著差异（95.69±3.60%比 94.26±4.84%）。总之，INRA96 方法更适合大熊猫精子冷冻保存，因此应推荐使用，以提高人工授精的成功率。
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