Lanza F, Mangianti S, Accorsi P, Lombardini L, Martino M, Saccardi R, Vassanelli A, Ostuni A, Ciceri F
Romagna Transplant Network, Ravenna, Italy.
Cell Processing Laboratory- PS-Cesena, Italy.
Transfus Apher Sci. 2020 Apr;59(2):102753. doi: 10.1016/j.transci.2020.102753. Epub 2020 Mar 5.
There is considerable heterogeneity in manipulation and cryopreservation of hematopoietic stem cells (HSC) for autologous HSC transplantation across Europe and Italy. To better address this point, three Italian Scientific Societies (GITMO- Gruppo Italiano per il Trapianto di Midollo Osseo; SIDEM- Società Italiana Emaferesi e Manipolazione Cellulare; and GIIMA- Gruppo Italiano Interdisciplinare Manipolazione e Aferesi per Terapie Cellulari), in collaboration with the Competent Authority "National Transplant Center" (CNT) sent to 85 Italian transplant centers (TC) a survey, which included 12 questions related to the most critical elements in graft processing. Fifty-nine centers (70 %) responded to the questionnaire. Overall, this survey demonstrates that the majority (>90 %) of responding TC used standardized procedures for HSC processing; however, an intercenter heterogeneity was clearly documented in several standard operating procedures adopted by different TC. These results seem to suggest that further standardization and efforts are needed to provide recommendations and guidelines on HSC manipulation, cryopreservation and functional assessment of cryopreserved material for autologous HSCT.
在欧洲和意大利，自体造血干细胞移植中造血干细胞（HSC）的处理和冷冻保存存在相当大的异质性。为了更好地解决这一问题，三个意大利科学学会（GITMO——意大利骨髓移植小组；SIDEM——意大利血液分离与细胞处理学会；以及GIIMA——意大利细胞治疗跨学科处理与血液分离小组）与主管机构“国家移植中心”（CNT）合作，向85个意大利移植中心（TC）发送了一份调查问卷，其中包括12个与移植物处理中最关键要素相关的问题。59个中心（70%）回复了问卷。总体而言，这项调查表明，大多数（>90%）回复的移植中心采用了标准化的HSC处理程序；然而，不同移植中心采用的几种标准操作程序中明显存在中心间异质性。这些结果似乎表明，需要进一步标准化并做出努力，以提供关于HSC处理、冷冻保存以及冷冻保存材料用于自体造血干细胞移植的功能评估的建议和指南。
