College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Hangzhou Wanxiang Polytechnic, Hangzhou 310023, China.
Bioresour Technol. 2020 Aug;309:123363. doi: 10.1016/j.biortech.2020.123363. Epub 2020 Apr 10.
The piggery digestate of high ammonia was mixed with the anoxic aerated effluent of high nitrate and phosphorus, to cultivate a microalgal-bacterial consortium for simultaneous pollution removal and resource recovery. The highest removal of total inorganic nitrogen was achieved at 324.77 mg/L in 40% piggery digestate mixed with 60% anoxic aerated effluent, along with the most microalgae biomass production. The crude protein and fatty acids of C14-C20 in microalgae cells were 21.80% and 69.78%, indicating that this mixing strategy could produce abundant microalgal biomass suitable for biofuel generation and animal feed. High-throughput sequencing showed that microbial diversity increased and Paenibacillus, Thiopseudomonas and Pseudomonas were the dominant species promoting microalgal growth. Overall, these results provided a new insight of mixing two types of wastewaters for cultivating microalgal-bacterial consortia, to remove contamination and recover nutrients simultaneously.
高氨猪粪与高硝氮和高磷缺氧曝气出水混合，培养微藻-细菌共生物，以实现同时去除污染和资源回收。在 40%猪粪和 60%缺氧曝气出水混合的条件下，总无机氮的去除率最高可达 324.77mg/L，同时微藻生物量的产量也最高。微藻细胞中的粗蛋白和 C14-C20 脂肪酸分别达到 21.80%和 69.78%，表明这种混合策略可以产生丰富的适合生物燃料生产和动物饲料的微藻生物质。高通量测序表明，微生物多样性增加，Paenibacillus、Thiopseudomonas 和 Pseudomonas 是促进微藻生长的主要优势种。总的来说，这些结果为利用两种废水混合培养微藻-细菌共生物，同时去除污染和回收营养物质提供了新的思路。
