Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea.
Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
Microbiome. 2022 Aug 31;10(1):142. doi: 10.1186/s40168-022-01339-3.
Monitoring microbial communities especially focused on pathogens in newly developed wastewater treatment systems is recommended for public health. Thus, we investigated the microbial community shift in a pilot-scale microalgal treatment system for piggery wastewater.
Microalgae showed reasonable removal efficiencies for COD and ammonia, resulting in higher transparency of the final effluent. Metagenome and microbial diversity analyses showed that heterotrophic microalgal cultivation barely changed the bacterial community; however, the mixotrophic microalgal cultivation induced a sudden change. In addition, an evaluation of risk groups (RGs) of bacteria showed that raw piggery wastewater included abundant pathogens, and the microalgal treatment of the raw piggery wastewater decreased the RG2 pathogens by 63%. However, co-cultivation of microalgae and the most dominant RG2 pathogen, Oligella, showed no direct effects between them.
Thus, a microbial interaction network was constructed to elucidate algae-bacteria interrelationships, and the decrease in Oligella was indirectly connected with microalgal growth via Brevundimonas, Sphingopyxis, and Stenotrophomonas. In a validation test, 3 among 4 connecting bacterial strains exhibited inhibition zones against Oligella. Therefore, we showed that microalgal wastewater treatment causes a decrease in RG2 bacteria, which is an indirect impact of microalgae associated with bacteria. Video abstract.
为了公共卫生，建议对新开发的污水处理系统中的微生物群落，尤其是病原体进行监测。因此，我们研究了猪粪废水处理的中试规模微藻处理系统中的微生物群落变化。
微藻对 COD 和氨氮表现出合理的去除效率，从而使最终出水的透明度更高。宏基因组和微生物多样性分析表明，异养微藻培养几乎没有改变细菌群落；然而，混合营养微藻培养引起了突然的变化。此外，对细菌风险组（RGs）的评估表明，原猪粪废水中含有丰富的病原体，微藻处理原猪粪废水可将 RG2 病原体减少 63%。然而，微藻与最主要的 RG2 病原体寡养单胞菌的共培养之间没有直接影响。
因此，构建了微生物相互作用网络来阐明藻类-细菌的相互关系，寡养单胞菌的减少与通过 Brevundimonas、Sphingopyxis 和 Stenotrophomonas 进行微藻生长间接相关。在验证测试中，连接细菌菌株中的 3 株对寡养单胞菌表现出抑制作用。因此，我们表明微藻废水处理会导致 RG2 细菌减少，这是与藻类相关的细菌的间接影响。视频摘要。
