Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, and Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 10080, China.
Shimadzu China Corporation, China MS Center, Beijing Branch, Beijing 100020, China.
Food Chem. 2020 Aug 30;322:126781. doi: 10.1016/j.foodchem.2020.126781. Epub 2020 Apr 9.
Triterpenic acids possess rich biological activity. Due to slight differences in structure and polarity, the simultaneous determination of isomeric triterpenic acids is challenging. In the present work, a simple and effective approach to chromatographic separation of such compounds based on conventional C18 stationary phase with gradient elution was developed, which allowed the simultaneous separation of eleven analytes including euscaphic, arjunic, tormentic, arjunolic, asiatic, pomolic, maslinic, corosolic, oleanolic, ursolic and 2-Epi tormentic acid (internal standard). This approach with mass spectrometric detection and ultrasonic extraction was fast, sensitive and accurate for analyzing isomeric triterpenic acids in O. fragrans fruits with a toal duration of the analytical cycle (including pretreatment) within one hour. The LODs lie in ranges of 0.8-12 ng/mL (30 ng/mL for asiatic and corosolic acid). The developed method was validated and successfully applied in ten batches of O. fragrans fruits, which could reflect the detail content difference of triterpenic acid components.
三萜酸具有丰富的生物活性。由于结构和极性略有差异，对其同分异构体的同时测定具有一定的挑战性。本工作建立了一种基于常规 C18 固定相梯度洗脱的简单有效的色谱分离方法，可同时分离 11 种分析物，包括羽扇豆酸、桃叶珊瑚酸、没食子酸、乌苏酸、齐墩果酸、熊果酸、马斯里酸、科罗索酸、橄榄酸、2-Epi 没食子酸（内标）。该方法结合质谱检测和超声提取，用于分析 O. fragrans 果实中的同分异构体三萜酸，分析周期（包括预处理）总时长在一小时内，快速、灵敏、准确。LOD 范围为 0.8-12ng/mL（齐墩果酸和科罗索酸为 30ng/mL）。该方法经过验证，并成功应用于 10 批 O. fragrans 果实，能够反映三萜酸成分的详细含量差异。
