Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and Wuhan University School of Pharmaceutical Sciences, Wuhan, China.
J Sep Sci. 2018 Nov;41(21):3995-4000. doi: 10.1002/jssc.201800610. Epub 2018 Sep 21.
A simple and sensitive high-performance liquid chromatography with fluorescence detection method has been developed for the determination of active components in Osmanthus fragrans fruits. In this work, reversed-phase liquid chromatography with a C18 column and mobile phase of methanol/water were applied to effectively separate three phenylethanoid glycosides, including salidroside, neonuezhenide, and nuezhenide. The limits of detection of salidroside, neonuezhenide, and nuezhenide were 3.9, 25.4, and 24.2 ng/mL respectively. Then this method was applied to determine the contents in several batches of O. fragrans fruits extract, which were collected from Wuhan, Hefei, and Changsha. Meanwhile, the method was used for the detection of rat plasma administrated with O. fragrans fruits extract, which could be an alternative method applied to the field of pharmacokinetics study in future. The results implied that the developed method was economical, simple, convenient, and sensitive, which was appropriate for the determination of salidroside, neonuezhenide, and nuezhenide in O. fragrans fruits.
建立了一种简单灵敏的反相高效液相色谱-荧光检测法，用于测定桂花果实中的活性成分。在这项工作中，采用甲醇/水作为流动相的 C18 柱反相液相色谱有效地分离了三种苯乙醇苷类化合物，包括红景天苷、新女贞苷和女贞苷。红景天苷、新女贞苷和女贞苷的检测限分别为 3.9、25.4 和 24.2ng/mL。然后，该方法用于测定来自武汉、合肥和长沙的几批桂花果实提取物中的含量。同时，该方法还用于检测大鼠血浆给予桂花果实提取物后的含量，这可能是未来在药代动力学研究领域应用的替代方法。结果表明，所建立的方法经济、简单、方便、灵敏，适用于桂花果实中红景天苷、新女贞苷和女贞苷的测定。
