Weinberger J M, Hawke M, Gotlieb A I
Department of Pathology, University of Toronto, Ontario, Canada.
J Otolaryngol. 1988 Dec;17(7):352-8.
Since the role of the epithelial cell in the repair of the wounded tympanic membrane is not well understood, the epithelial cell layers were examined using rhodamine phalloidin to localize F-actin in situ following a full-thickness traumatic perforation. The change in shape of the epithelial cells and the morphological changes in F-actin were characterized. The mucosal cells were remarkably resistant to wounding and their morphology and F-actin distribution remained unchanged. Within 24 hours following perforation, basal cells were prominently stained adjacent to the perforation, whereas the remainder of the drum showed fainter staining similar to non-wounded drums. The basal cells showed a minor shape change in the direction of movement of the surface keratin. By three days, the dense peripheral F-actin staining of basal cells was prominent throughout the entire drum. The suprabasal cells demonstrated a marked shape change by 24 hours following the injury. The normal cobblestone pattern disappeared and cells elongated and were aligned towards the perforation. This occurred first adjacent to the perforation, and by three days re-orientation was present over the entire tympanic membrane. A few fine stress fibers appeared in the suprabasal cells. Histological and electron microscopic evaluation of the areas away from the perforation did not, however, show inflammation or disruption. By one week these changes were resolving and by two weeks the drum, with the exception of the cells in the area of the healed perforation, had returned to normal. The results are discussed with respect to the role of the various cell types in the migratory aspect of tympanic membrane wound repair.
由于上皮细胞在鼓膜创伤修复中的作用尚未完全明确，因此在鼓膜全层创伤穿孔后，使用罗丹明鬼笔环肽对上皮细胞层进行原位检查，以定位F-肌动蛋白。对上皮细胞的形状变化和F-肌动蛋白的形态变化进行了表征。黏膜细胞对创伤具有显著的抵抗力，其形态和F-肌动蛋白分布保持不变。穿孔后24小时内，穿孔附近的基底细胞染色明显，而鼓膜其余部分的染色与未受伤的鼓膜相似，较淡。基底细胞在表面角质形成细胞移动方向上有轻微的形状变化。到第三天，基底细胞密集的外周F-肌动蛋白染色在整个鼓膜上都很明显。损伤后24小时，基底上层细胞出现明显的形状变化。正常的鹅卵石样图案消失，细胞伸长并朝着穿孔方向排列。这种情况首先出现在穿孔附近，到第三天，整个鼓膜都出现了重新定向。基底上层细胞中出现了一些细的应力纤维。然而，对穿孔区域以外的区域进行组织学和电子显微镜评估，未发现炎症或破坏。到一周时，这些变化开始消退，到两周时，除愈合穿孔区域的细胞外，鼓膜已恢复正常。讨论了各种细胞类型在鼓膜伤口修复迁移方面的作用的结果。
