Division of General Surgery and Trauma, Department of Surgery, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo (HC/FMUSP), Dr. Enéas de Carvalho Aguiar Av. 255. Cerqueira Cesar, São Paulo, 05402-000, Brazil.
, Baturite St, 120. Ap 91. Aclimação, São Paulo, 01530-030, Brazil.
Hernia. 2020 Dec;24(6):1325-1336. doi: 10.1007/s10029-020-02186-7. Epub 2020 Apr 18.
To evaluate the vas deferens and testicles of rats submitted to bilateral inguinotomy and polypropylene (PP) mesh placement.
Sixty Wistar rats were randomized into three groups: Control (inguinotomy only), mesh placement over the vas deferens (Mesh-DD) or under the spermatic funiculus (Mesh-SF). The following analyses were performed: vas deferens morphometry (lumen area and wall thickness), quantification of collagen fibers, spermatogenesis, apoptosis (cleaved caspase-3 and TUNEL) and cellular proliferation (Ki67). Quantitative gene expression (qPCR) for apoptosis and inflammatory cytokines were evaluated by RT-PCR.
In the apoptosis pathway, Mesh-DD showed one upregulated gene (Il10) and three downregulated genes (Fadd, Tnfrsf1b and Xiap). In Mesh-SF, 17 genes were downregulated. In the inflammation pathway (Mesh-DD), one gene was upregulated (Il1r1), and one gene was downregulated (Ccl12). In Mesh-SF, three genes were upregulated (Il1r1, Tnfsf13b and Csf1), and two were downregulated (Ccl12 and Csf2). PP mesh placement preserved spermatogenesis and did not alter the vas deferens or the testicle. In the ductus deferens, there was reduced luminal area (30 days), increased wall thickness (90 days), and increased type III collagen and cell proliferation (30 and 90 days) (p < 0.05). In the testicle, cell proliferation was greater in the Mesh-DD (p < 0.05).
PP mesh, whether or not in direct contact with spermatic funicular structures, induces changes that were not sufficient to cause damage to the evaluated organs.
评估双侧腹股沟切开术和聚丙烯（PP）网片放置后大鼠的输精管和睪丸。
将 60 只 Wistar 大鼠随机分为三组：对照组（仅行腹股沟切开术）、输精管上方放置网片（Mesh-DD）或精索下方放置网片（Mesh-SF）。进行以下分析：输精管形态学（管腔面积和管壁厚度）、胶原纤维定量、精子发生、细胞凋亡（cleaved caspase-3 和 TUNEL）和细胞增殖（Ki67）。通过 RT-PCR 评估细胞凋亡和炎症细胞因子的定量基因表达（qPCR）。
在细胞凋亡途径中，Mesh-DD 显示一个上调基因（Il10）和三个下调基因（Fadd、Tnfrsf1b 和 Xiap）。在 Mesh-SF 中，17 个基因下调。在炎症途径中（Mesh-DD），一个基因上调（Il1r1），一个基因下调（Ccl12）。在 Mesh-SF 中，三个基因上调（Il1r1、Tnfsf13b 和 Csf1），两个基因下调（Ccl12 和 Csf2）。PP 网片放置保留了精子发生，并且不改变输精管或睪丸。在输精管中，管腔面积减少（30 天），管壁厚度增加（90 天），并且 III 型胶原和细胞增殖增加（30 和 90 天）（p<0.05）。在睪丸中，Mesh-DD 的细胞增殖更大（p<0.05）。
PP 网片，无论是否直接接触精索结构，都会引起变化，但不足以对评估的器官造成损害。
