Department for Molecular and Cellular Sports Medicine, Institute for Cardiovascular Research and Sports Medicine, German Sport University Cologne, Cologne, Germany.
Center for Preventive Doping Research/Institute of Biochemistry, German Sport University Cologne, Cologne, Germany.
Eur J Appl Physiol. 2020 Jun;120(6):1429-1436. doi: 10.1007/s00421-020-04375-9. Epub 2020 Apr 18.
Due to distinct immuno- and neuro-modulatory properties, growing research interest focuses on exercise-induced alterations of the kynurenine (KYN) pathway in healthy and clinical populations. To date, knowledge about the impact of different acute strength exercise modalities on the KYN pathway is scarce. Therefore, we investigated the acute effects of hypertrophic (HYP) compared to maximal (MAX) strength loadings on the KYN pathway regulation.
Blood samples of twelve healthy males (mean age and weight: 23.5 ± 3.2 years; 77.5 ± 7.5 kg) were collected before (T), immediately after (T), and 1 h after completion (T) of HYP (5 sets with 10 repetitions at 80% of 1RM) and MAX (15 sets with 1RM) loadings performed in a randomized cross-over design. Serum concentrations of tryptophan (TRP), KYN, kynurenic acid (KA), and quinolinic acid (QA) were assessed using high-performance liquid chromatography.
The KA/KYN ratio increased from T to T (p = 0.01) and decreased from T to T (p = 0.011) in HYP, while it was maintained within MAX. Compared to MAX, serum concentrations of KA were greater in HYP at T (p = 0.014). Moreover, the QA/KA ratio was significantly lower in HYP than in MAX at T (p = 0.002).
Acute HYP loading led to increases in the metabolic flux yielding KA, thereby possibly promoting immunosuppression and neuroprotection. Our findings emphasize the potential of acute HYP exercise as short-term modulator of KYN pathway downstream to KA in healthy males and need to be proven in other samples.
由于具有独特的免疫和神经调节特性，越来越多的研究关注于运动对健康人群和临床人群中犬尿氨酸（KYN）途径的影响。迄今为止，关于不同急性力量运动方式对 KYN 途径调节的影响的知识还很匮乏。因此，我们研究了与最大力量负荷相比，肥大（HYP）力量负荷对 KYN 途径调节的急性影响。
12 名健康男性（平均年龄和体重：23.5±3.2 岁；77.5±7.5kg）参与了这项随机交叉设计的研究。在 HYP（5 组，每组 10 次，重复 80%的 1RM）和 MAX（15 组，每组 1RM）负荷完成前（T）、即刻（T）和完成后 1 小时（T）采集血液样本。采用高效液相色谱法检测色氨酸（TRP）、犬尿氨酸（KYN）、犬尿氨酸酸（KA）和喹啉酸（QA）的血清浓度。
HYP 中 KA/KYN 比值从 T 增加到 T（p=0.01），从 T 降低到 T（p=0.011），而在 MAX 中则保持不变。与 MAX 相比，HYP 在 T 时血清 KA 浓度更高（p=0.014）。此外，HYP 在 T 时的 QA/KA 比值明显低于 MAX（p=0.002）。
急性 HYP 负荷导致产生 KA 的代谢通量增加，从而可能促进免疫抑制和神经保护。我们的发现强调了急性 HYP 运动作为健康男性中 KA 下游 KYN 途径的短期调节剂的潜力，需要在其他样本中得到证实。
