Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
J Mol Neurosci. 2020 Jun;70(6):871-877. doi: 10.1007/s12031-020-01511-0. Epub 2020 Apr 18.
MicroRNAs (miRNAs) are considered among the most reliable biomarkers to diagnose and predict Alzheimer's disease (AD), due to their regulatory nature. The main goal of this study was to evaluate the expression of miR4422 and miR3714, as the main regulators of GSAP and BACE1 expression, in AD patients compared with healthy subjects. Twenty patients with a mild to moderate AD (58-71 years old) and 15 healthy subjects (58-73 years old) participated in this study. The expression levels of miR4422 and miR3714 as the target genes and 5S rRNA and miRlet7a-5p as the reference genes were measured in the two groups. To compare the expression between the case and the control groups, the t test or the Wilcoxon test was used, based on the data distribution patterns. The efficiencies of amplification of the miR4422, miR3714, 5S rRNA, and miRlet7a-5p genes all were in the acceptable range. The mean miR4422-5S rRNA dCt value was significantly different between the two groups (p = 0.018). The relative fold change of the expression was 0.43. The mean miR4422-miRlet7a-5p dCt value (p = 0.41), the mean miR3714-5S rRNA dCt value (p = 0.10), and the mean miR3714-miRlet7a-5p dCt value (p = 0.063) were not significantly different between the two groups. We indicated that miR4422 could be a reliable biomarker for Alzheimer's diagnosis. It seems that the reduced expression of miR4422 that targets GSAP and BACE1 expression can lead to an increase in the formation of Aβ plaque.
microRNAs (miRNAs) 被认为是诊断和预测阿尔茨海默病 (AD) 最可靠的生物标志物之一，这是由于它们的调节性质。本研究的主要目的是评估 miR4422 和 miR3714 的表达，作为 GSAP 和 BACE1 表达的主要调节剂，在 AD 患者与健康受试者中的表达。20 名轻度至中度 AD 患者（58-71 岁）和 15 名健康受试者（58-73 岁）参加了这项研究。测量了两组中 miR4422 和 miR3714 作为靶基因和 5S rRNA 和 miRlet7a-5p 作为参照基因的表达水平。为了比较病例组和对照组之间的表达，根据数据分布模式，使用 t 检验或 Wilcoxon 检验。miR4422、miR3714、5S rRNA 和 miRlet7a-5p 基因的扩增效率均在可接受范围内。两组之间 miR4422-5S rRNA dCt 值的平均值差异有统计学意义（p=0.018）。表达的相对倍数变化为 0.43。miR4422-miRlet7a-5p dCt 值的平均值（p=0.41）、miR3714-5S rRNA dCt 值的平均值（p=0.10）和 miR3714-miRlet7a-5p dCt 值的平均值（p=0.063）在两组之间差异无统计学意义。我们表明，miR4422 可能是 AD 诊断的可靠生物标志物。似乎 miR4422 的表达减少，靶向 GSAP 和 BACE1 表达，会导致 Aβ 斑块形成增加。
