通过“凝集素抑制”测定法对糖型进行简单快速的检测。
Simple and Rapid Detection of Glycoforms by "Lectin Inhibition" Assay.
机构信息
Department of Biochemistry, Fukushima Medical University, Fukushima, Japan.
Department of Human Life Science, Fukushima Medical University School of Nursing, Fukushima, Japan.
出版信息
Methods Mol Biol. 2020;2132:165-171. doi: 10.1007/978-1-0716-0430-4_17.
Glycoforms are otherwise identical proteins with different glycosylation. A lectin, Sambucus sieboldiana agglutinin (SSA), specifically binds glycoforms having α2,6-sialyl residues. The binding is found to inhibit antigen-antibody reaction; e.g., SSA inhibits anti-transferrin antibody binding to α2,6-sialylated transferrin (Tf) (SSA inhibition). SSA inhibition is not observed with other Tf glycoforms, indicating that the inhibition is glycoform-specific. Here we describe the application of SSA inhibition to ELISA as a specific assay for quantifying α2,6-sialylated Tf.
糖型是指具有不同糖基化的相同蛋白质。凝集素，接骨木凝集素（SSA），特异性结合具有α2,6-唾液酸残基的糖型。已发现该结合抑制抗原-抗体反应；例如，SSA 抑制抗转铁蛋白抗体与α2,6-唾液酸化转铁蛋白（Tf）（SSA 抑制）的结合。其他 Tf 糖型不会观察到 SSA 抑制，表明抑制是糖型特异性的。本文描述了 SSA 抑制在 ELISA 中的应用，作为定量检测α2,6-唾液酸化 Tf 的特异性检测方法。
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