通过“凝集素抑制”进行免疫组化中的糖型特异性可视化。

Glycoform-Specific Visualization in Immunohistochemistry by "Lectin Inhibition".

机构信息

Department of Biochemistry, Fukushima Medical University, Fukushima, Japan.

Department of Human Life Science, Fukushima Medical University School of Nursing, Fukushima, Japan.

出版信息

Methods Mol Biol. 2020;2132:173-181. doi: 10.1007/978-1-0716-0430-4_18.

DOI:10.1007/978-1-0716-0430-4_18

PMID:32306326

Abstract

Antibodies are useful for localizing glycoprotein antigens in histochemistry, but they do not differentiate glycoforms in tissue sections because conventional antibodies recognize only protein epitopes rather than glycans. Glycan epitopes are recognized by lectins, which are found, occasionally, to inhibit antigen-antibody reaction in a glycoform-specific manner (lectin inhibition). Here we describe the application of lectin inhibition to immunohistochemistry for visualizing a glycoform in a tissue section.

摘要

抗体在组织化学中用于定位糖蛋白抗原，但它们不能区分组织切片中的糖型，因为传统抗体仅识别蛋白质表位而不是聚糖。糖基表位被凝集素识别，凝集素偶尔以糖型特异性方式抑制抗原-抗体反应（凝集素抑制）。在这里，我们描述了凝集素抑制在免疫组织化学中用于可视化组织切片中糖型的应用。