糙皮侧耳菌丝凝集素（PCL-M）：活性的菌丝体表面检测与纯化。

Pleurotus cornucopiae Mycelial Lectin (PCL-M): Purification and Detection of the Activity on Mycelial Surface.

机构信息

Department of Northern Biosphere Agriculture, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.

出版信息

Methods Mol Biol. 2020;2132:445-452. doi: 10.1007/978-1-0716-0430-4_43.

DOI:10.1007/978-1-0716-0430-4_43

PMID:32306351

Abstract

Pleurotus cornucopiae mycelial lectin (PCL-M) is a divalent cation-dependent GalNAc-specific lectin that is purified from the mycelia of the edible mushroom Pleurotus cornucopiae. PCL-M is produced only in dikaryotic mycelia growing on solid medium and is not present in the mycelia of fruiting bodies. It is a multimeric glycoprotein composed of 40 kDa subunits linked by disulfide bonds. PCL-M is localized on the surface of aggregating mycelia and may be involved in primordia formation. The procedure for PCL-M purification has been discussed herein. Additionally, an experimental method for analyzing the localization of PCL-M on mycelial surface has been described.

摘要

姬松茸菌丝体凝集素（PCL-M）是一种二价阳离子依赖性半乳糖胺特异性凝集素，从可食用蘑菇姬松茸的菌丝体中纯化得到。PCL-M 仅在生长在固体培养基上的双核菌丝体中产生，而不在子实体的菌丝体中存在。它是一种由 40 kDa 亚基组成的多聚体糖蛋白，通过二硫键连接。PCL-M 位于聚集菌丝体的表面，可能参与原基形成。本文讨论了 PCL-M 的纯化程序。此外，还描述了一种用于分析 PCL-M 在菌丝体表面定位的实验方法。