College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China.
Microbiol Res. 2021 Jun;247:126723. doi: 10.1016/j.micres.2021.126723. Epub 2021 Feb 14.
Hydrophobins are small secreted proteins with important physiological functions and potential applications. Here, Pleurotus ostreatus hydrophobin genes were systematically analyzed: they were characterized, classified, and their expression profiles and gene functions were explored. In total, 40 P. ostreatus hydrophobin genes were found and showed genetic diversity, of which 15 were newly identified. The hydrophobin protein sequences were diverse but all contained eight cysteine residues with a conserved spacing pattern, and 33 of them were class I hydrophobins. The expression profile analyses showed that Vmh3 and Hydph20 were abundant in monokaryotic and dikaryotic mycelia, whereas Hydph17, Po.hyd16, Hydph8 were specifically expressed in monokaryotic mycelia and Po.hyd10 were specific in dikaryotic mycelia. Furthermore, Vmh3, Hydph20, Po.hyd7, and Po.hyd10 were abundant when dikaryotic mycelia cultivated on PDA, which are different from on substrate (Vmh2, Vmh3, Hydph7, Po.hyd3, Po.hyd7, Po.hyd9); Hydph12, POH1, and Po.hyd4 can be induced by natural light and cold stimulation during development from mycelia to primordia; Vmh3, FBH1, Hydph12, Po.hyd1-Po.hyd5, and Po.hyd8 were highly expressed in primordia and young fruiting bodies; Hydph12, Po.hyd1, Po.hyd4, and Po.hyd5 were specifically expressed in pilei. In addition, RNAi transformants of FBH1 exhibited slower growth rates and had fewer primordia and fruiting bodies, which suggests FBH1 affects the growth rate and primordia formation of P. ostreatus. Therefore, P. ostreatus hydrophobin genes belong to a large family and are temporally and spatially expressed to meet the developmental needs of mushroom.
水蛋白是具有重要生理功能和潜在应用价值的小分泌蛋白。本文系统分析了糙皮侧耳水蛋白基因：对其特征、分类、表达谱和基因功能进行了探讨。共发现 40 个糙皮侧耳水蛋白基因，具有遗传多样性，其中 15 个为新鉴定的基因。水蛋白蛋白序列多样，但均含有 8 个半胱氨酸残基，且具有保守的间隔模式，其中 33 个属于 I 类水蛋白。表达谱分析表明，Vmh3 和 Hydph20 在单核和双核菌丝中丰富表达，而 Hydph17、Po.hyd16、Hydph8 则在单核菌丝中特异性表达，Po.hyd10 则在双核菌丝中特异性表达。此外，当双核菌丝在 PDA 上培养时，Vmh3、Hydph20、Po.hyd7 和 Po.hyd10 表达丰富，这与在基质上（Vmh2、Vmh3、Hydph7、Po.hyd3、Po.hyd7、Po.hyd9）不同；Hydph12、POH1 和 Po.hyd4 可以在菌丝体向原基发育过程中被自然光和冷刺激诱导；Vmh3、FBH1、Hydph12、Po.hyd1-Po.hyd5 和 Po.hyd8 在原基和幼果体中高度表达；Hydph12、Po.hyd1、Po.hyd4 和 Po.hyd5 在菌盖上特异性表达。此外，FBH1 的 RNAi 转化体生长速度较慢，原基和果体较少，这表明 FBH1 影响糙皮侧耳的生长速度和原基形成。因此，糙皮侧耳水蛋白基因属于一个大家族，并且在时间和空间上表达，以满足蘑菇发育的需要。
