Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
J Phycol. 2020 Oct;56(5):1208-1215. doi: 10.1111/jpy.13007. Epub 2020 May 26.
Survival of coral reef-associated Symbiodiniaceae is vital to maintain the healthy coral community in coral reefs. However, knowledge about cultivation of free-living or symbiotic Symbiodiniaceae has been limited. In this study, the response surface methodology was applied to optimize the medium for Effrenium voratum. The results showed that the impacts of nutrient components on algal growth were: FeCl > NaH PO >MnSO > MgSO /CoSO > KCl>ZnSO > CaCl /NaNO , among which NaH PO and FeCl significantly affected algal growth. The optimal medium was: natural seawater supplemented with NaH PO ·2H O 0.25 mM,FeCl ·6H O 14.24 μM, NaNO 0.94 mM, MgSO ·7H O 40.63 mM, KCl 5.37 mM, CaCl ·2H O 4.08 mM, ZnSO ·7H O 0.35 μM, MnSO 9.93 μM, and CoSO 0.36 μM. The use of the optimized medium resulted in an increase of biomass yield (0.76 g dry weight · L ) by 46% over that using the initial medium, which agreed with the predicted value (0.71 g · L ). Additionally, fatty acids, mainly consisting of palmitic acid (C16:0) and ethyl carbonate (C20:0), accounted for approximately 50% of the total fatty acids in E. voratum. Interestingly, docosahexaenoic acid (DHA) accounted for 6% of total fatty acids, a high proportion that makes E. voratum a potential candidate feedstock in aquaculture for DHA production.
珊瑚礁共生共生体 Symbiodiniaceae 的生存对于维持珊瑚礁健康的珊瑚群落至关重要。然而，关于自由生活或共生共生体 Symbiodiniaceae 的培养知识一直有限。在这项研究中，响应面法被应用于优化 Effrenium voratum 的培养基。结果表明，营养成分对藻类生长的影响为：FeCl > NaHPO > MnSO > MgSO/CoSO > KCl> ZnSO > CaCl/NaNO ，其中 NaHPO 和 FeCl 显著影响藻类生长。最佳培养基为：天然海水补充 0.25mM 的 NaHPO·2H O、14.24μM 的 FeCl·6H O、0.94mM 的 NaNO、40.63mM 的 MgSO·7H O、5.37mM 的 KCl、4.08mM 的 CaCl·2H O、0.35μM 的 ZnSO·7H O、9.93μM 的 MnSO 和 0.36μM 的 CoSO。与初始培养基相比，优化后的培养基使生物量产量（0.76g 干重·L）增加了 46%，这与预测值（0.71g·L）相符。此外，脂肪酸主要由棕榈酸（C16:0）和碳酸乙酯（C20:0）组成，约占 E. voratum 总脂肪酸的 50%。有趣的是，二十二碳六烯酸（DHA）占总脂肪酸的 6%，这一高比例使 E. voratum 成为水产养殖中生产 DHA 的潜在候选饲料。
