Key Laboratory of Biomedical Information Engineering of Education Ministry, Institute of Biomedical Analytical Technology and Instrumentation, School of life Science and Technology, Xi'an Jiaotong University, Xi'an, China.
Institute of Biomedical Optics, University of lübeck, Lübeck, Germany.
J Biophotonics. 2020 Jul;13(7):e202000017. doi: 10.1002/jbio.202000017. Epub 2020 May 5.
The delivery of macromolecules into living cells is challenging since in most cases molecules are endocytosed and remain in the endo-lysosomal pathway where they are degraded before reaching their target. Here, a method is presented to selectively improve cell membrane permeability by nanosecond laser irradiation of gold nanorods (GNRs) with visible or near-infrared irradiation in order to deliver proteins across the plasma membrane, avoiding the endo lysosomal pathway. GNRs were labeled with the anti-EGFR (epidermal growth factor receptor) antibody Erbitux to target human ovarian carcinoma cells OVCAR-3. Irradiation with nanosecond laser pulses at wavelengths of 532 nm or 730 nm is used for transient permeabilization of the cell membranes. As a result of the irradiation, the uptake of an anti-Ki-67 antibody was observed in about 50 % of the cells. The results of fluorescence lifetime imaging show that the GNR detached from the membrane after irradiation.
将大分子递送到活细胞中具有挑战性，因为在大多数情况下，分子被内吞作用并停留在内体溶酶体途径中，在到达靶标之前被降解。在这里，提出了一种通过纳秒激光辐照金纳米棒（GNR）来选择性提高细胞膜通透性的方法，该方法使用可见光或近红外辐射，以便将蛋白质递送到质膜中，从而避免内体溶酶体途径。GNR 用抗 EGFR（表皮生长因子受体）抗体 Erbitux 标记，以靶向人卵巢癌细胞 OVCAR-3。使用波长为 532nm 或 730nm 的纳秒激光脉冲进行辐照，以瞬时通透细胞膜。辐照后，约 50%的细胞中观察到抗 Ki-67 抗体的摄取。荧光寿命成像的结果表明，辐照后 GNR 从膜上脱离。
