Zhou W H, Gu G Z, Wu H, Li Y H, Chen G S, Zhang H L, Yu S F, Zheng Y X
Henan Provincial Institute for Occupational Health, Zhengzhou 450052, China.
Wugang Institute for Occupational Health, Wugang 462599, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2020 Feb 20;38(2):111-116. doi: 10.3760/cma.j.issn.1001-9391.2020.02.007.
The purpose of this study was to explore the association between gene in the potassium recycling pathway 4 (KCNQ4) polymorphisms and the susceptibility to noise-induced hearing loss (NIHL) , and analysis the effect of cumulative noise exposure (CNE) and noise exposure duration on this association. A nested case-control study with 1∶1 matched was used based on the cohort of noise exposure in a steel factory. A total of 286 cases were selected as the group of hearing loss and 286 controls were chosen according to the matching standards of same gender, same type of work, age difference ≤ 5 years, noise exposure duration ≤ 2 years. The single nucleotide polymorphisms (SNPs) of rs4660468, rs4660470, rs34287852 in KCNQ4 were genotyped by SNPscan(TM) method. The codominant, dominant and recessive models were established to study KCNQ4 polymorphisms and the susceptibility to NIHL by single-factor conditional logistic regression analysis. The COX regression analysis was used to analyze the risk of developing NIHL in individuals with different genotypes along with the extending of noise exposure duration or CNE. In the case of CNE≤96 dB (A) ·year, the risk of developing NIHL in individuals with TA genotype of rs4660470 was 2.197 times than individuals with TT genotypes (95%: 1.0324.677) , and those with TA+AA and TT genotypes (HR=2.467, 95%: 1.0255.934) With the increase of noise exposure duration, in rs4660470, individuals with TA genotype had a higher risk of suffering NIHL than those with TT genotype (HR=1.461, 95%: 1.061~2.011) , individuals with TA and/or AA genotype had a earlier risk of suffering NIHL than those with TT genotype. The mutant allele A of rs4660470 in KCNQ4 may be a risk factor for developing NIHL, CNE≤100 dB (A) ·year or the increase of noise exposure duration may further increase the risk of NIHL.
本研究旨在探讨钾离子循环通路4（KCNQ4）基因多态性与噪声性听力损失（NIHL）易感性之间的关联，并分析累积噪声暴露（CNE）和噪声暴露持续时间对这种关联的影响。基于某钢铁厂噪声暴露队列，采用1∶1匹配的巢式病例对照研究。共选取286例听力损失患者作为病例组，按照性别相同、工作类型相同、年龄相差≤5岁、噪声暴露持续时间≤2年的匹配标准选取286例对照。采用SNPscan(TM)方法对KCNQ4基因的rs4660468、rs4660470、rs34287852单核苷酸多态性（SNP）进行基因分型。通过单因素条件logistic回归分析建立共显性、显性和隐性模型，研究KCNQ4基因多态性与NIHL易感性的关系。采用COX回归分析，分析不同基因型个体随着噪声暴露持续时间延长或CNE增加发生NIHL的风险。在CNE≤96 dB（A）·年的情况下，rs4660470基因TA基因型个体发生NIHL的风险是TT基因型个体的2.197倍（95%：1.0324.677），TA+AA基因型个体与TT基因型个体相比（HR=2.467，95%：1.0255.934）。随着噪声暴露持续时间增加，在rs4660470基因中，TA基因型个体发生NIHL的风险高于TT基因型个体（HR=1.461，95%：1.061~2.011），TA和/或AA基因型个体发生NIHL的风险早于TT基因型个体。KCNQ4基因rs4660470的突变等位基因A可能是发生NIHL的危险因素，CNE≤100 dB（A）·年或噪声暴露持续时间增加可能会进一步增加NIHL的风险。
