鹅基因的分子克隆及与产蛋性状相关多态性的鉴定。

Molecular cloning of the goose gene and identification of polymorphisms associated with laying traits.

Poultry Science Department, Chongqing Academy of Animal Science , Chongqing, China.

Poultry Science Department, Chongqing Engineering Research Center of Goose Genetic Improvement , Chongqing, China.

Br Poult Sci. 2020 Oct;61(5):502-507. doi: 10.1080/00071668.2020.1758298. Epub 2020 May 27.

Abstract

Egg-laying traits are important economic characteristics in goose production (). The gene , which encodes gonadotropin-releasing hormone, is a strong candidate gene for egg-laying traits in avian species. 2. In this study, a 3520 bp genomic sequence and a 279 bp mRNA sequence for , which encoded 92 amino acids, were determined. The DNA sequence contains four exons and three introns, and the DNA and deduced amino acid sequences were highly conserved across mammals (human, macaque, cow, and sheep) and avians (chicken, fulmar and quail). 3. Using a direct sequencing method, 46 single nucleotide polymorphisms (SNPs) were identified in the genomic sequence that were shared between two Sichuan White goose populations (217 and 208 individuals). Furthermore, 44 haplotypes were constructed using a sliding window approach. Association analysis between the SNPs and haplotypes and egg-laying traits showed that 10 SNPs affected the first egg weight, average egg weight, egg number at 48 weeks and egg number at 64 weeks. 4. These results lay the foundation for further studies of the function of in geese and provide a theoretical basis for marker-assisted selection of egg-laying traits in the Sichuan white goose population.

摘要

产蛋性状是鹅生产中的重要经济特征（）。编码促性腺激素释放激素的基因是禽类产蛋性状的候选基因。
在这项研究中，确定了 3520 bp 的基因组序列和 279 bp 的 mRNA 序列（），其编码 92 个氨基酸。DNA 序列包含四个外显子和三个内含子，并且 DNA 和推导的氨基酸序列在哺乳动物（人类、猕猴、牛和绵羊）和禽类（鸡、管鼻鹱和鹌鹑）中高度保守。
使用直接测序方法，在两个四川白鹅群体（217 和 208 个个体）之间的基因组序列中鉴定出 46 个单核苷酸多态性（SNP）。此外，使用滑动窗口方法构建了 44 个单倍型。SNP 和单倍型与产蛋性状的关联分析表明，10 个 SNP 影响了初产蛋重、平均蛋重、48 周产蛋数和 64 周产蛋数。
这些结果为进一步研究在鹅中的功能奠定了基础，并为四川白鹅群体产蛋性状的标记辅助选择提供了理论依据。