Matheson Austyn, Regmi Suresh C, Martin-Alarcon Leonardo, Jay Gregory D, Scott W Michael, Schmidt Tannin A
Biomedical Engineering Graduate Program, University of Calgary, Calgary, AB, Canada.
Department of Emergency Medicine - Warren Alpert Medical School & School of Engineering, Brown University, Providence, RI, USA.
Connect Tissue Res. 2021 Jul;62(4):369-380. doi: 10.1080/03008207.2020.1751140. Epub 2020 Apr 20.
: In experimental models of equine joint-injury and osteoarthritis synovial fluid (SF) composition (proteoglycan-4, hyaluronan) can vary, along with changes to SF mechanical function (lubrication, viscosity). The study hypotheses were a) clinical equine joint-injury and disease results in altered SF composition and diminished mechanical function, and b) serum composition (proteoglycan-4 or hyaluronan) changes concurrently. The objectives were to characterize composition (proteoglycan-4, hyaluronan), and function of SF and serum from normal horses compared to clinical groups: osteoarthritis, acute-joint-injury, and osteochondrosis.: Equine samples of SF (from various joints) and blood were collected at the point-of-care. Proteoglycan-4 concentrations were measured by amplified-luminescence-proximity-assay and enzyme-linked-immunosorbent-assay in SF and serum, respectively. Molecular-weight of hyaluronan was characterized by agarose-gel-electrophoresis, and concentrations were measured by enzyme-linked-immunosorbent-assay kit. Biomechanical function of SF was characterized by an cartilage-on-cartilage friction test, and viscosity test.: SF proteoglycan-4 concentration increased in acute-joint-injury (1185 ± 276 versus normal 205 ± 106 µg/mL, µ± SEM, p < 0.01), with increased percentage of lower molecular-weight hyaluronan in acute-joint-injury and osteochondrosis. SF and serum proteoglycan-4 concentrations were correlated in normal horses (r = 0.85, p < 0.05), but not in clinical groups. Cartilage-lubricating ability was unchanged, although steady-shear viscosity of acute-joint-injury SF decreased from normal.: Composition of SF from cases of equine acute-joint-injury changed; both proteoglycan-4 concentration and hyaluronan molecular-weight were altered, with decreased SF viscosity, but no associated changes to serum. Serum proteoglycan-4 and hyaluronan concentrations alone may not be useful biomarkers for equine joint-injury or disease.
在马关节损伤和骨关节炎的实验模型中，滑液（SF）的成分（蛋白聚糖-4、透明质酸）会发生变化，同时SF的机械功能（润滑、粘度）也会改变。研究假设为：a）临床马关节损伤和疾病会导致SF成分改变以及机械功能减弱；b）血清成分（蛋白聚糖-4或透明质酸）会同时发生变化。目的是对比正常马与临床组（骨关节炎、急性关节损伤和骨软骨病）的SF和血清的成分（蛋白聚糖-4、透明质酸）及功能。
在护理现场采集马的SF（来自不同关节）和血液样本。分别通过放大发光邻近分析法和酶联免疫吸附测定法测量SF和血清中的蛋白聚糖-4浓度。通过琼脂糖凝胶电泳表征透明质酸的分子量，并使用酶联免疫吸附测定试剂盒测量其浓度。通过软骨对软骨摩擦试验和粘度试验表征SF的生物力学功能。
急性关节损伤时SF中蛋白聚糖-4浓度升高（1185±276与正常的205±106μg/mL，均值±标准误，p<0.01），急性关节损伤和骨软骨病中低分子量透明质酸的百分比增加。正常马中SF和血清蛋白聚糖-4浓度相关（r=0.85，p<0.05），但临床组中不相关。尽管急性关节损伤SF的稳态剪切粘度比正常时降低，但软骨润滑能力未改变。
马急性关节损伤病例的SF成分发生变化；蛋白聚糖-4浓度和透明质酸分子量均改变，SF粘度降低，但血清无相关变化。仅血清蛋白聚糖-4和透明质酸浓度可能不是马关节损伤或疾病的有用生物标志物。
