von Volkmann Hilde L, Brønstad Ingeborg, Tronstad Rune R, Dizdar Vernesa, Nylund Kim, Hanevik Kurt, Hausken Trygve, Gilja Odd Helge, Fiskerstrand Torunn
National Centre for Ultrasound in Gastroenterology, Department of Medicine, Haukeland University Hospital.
Department of Clinical Medicine, University of Bergen.
Scand J Gastroenterol. 2020 Apr;55(4):449-453. doi: 10.1080/00365521.2020.1748224. Epub 2020 Apr 20.
Guanylin (GN) and uroguanylin (UGN) are endogenous ligands for the intestinal receptor guanylate cyclase C (GC-C), an important regulator of intestinal fluid homeostasis. Gene expression and protein levels of GN are suppressed in inflamed intestinal tissue from patients with inflammatory bowel disease (IBD), but knowledge about plasma levels of guanylins in these conditions is sparse. We aimed to investigate the fasting plasma levels of the prohormones proGN and proUGN in patients with Crohn's Disease (CD) and relate these to levels found in persons with other diarrheal conditions, as well as persons with normal bowel habits. Plasma from patients with CD, patients with Familial Diarrheal Disease (FGDS), diarrhea-predominant irritable bowel syndrome (IBS-D) and healthy controls (HC) was analyzed using ELISA assays. Significantly lower fasting plasma levels of proguanylins were found in CD and FGDS patients, compared to HC. In CD patients, plasma proGN levels correlated negatively with Harvey Bradshaw Index and with number of stools/24 h. Our data indicate that diarrhea may be a determinant for levels of proGN in plasma, and should be further explored in studies of different diarrheal disorders.
鸟苷林（GN）和尿鸟苷林（UGN）是肠道受体鸟苷酸环化酶C（GC - C）的内源性配体，而GC - C是肠道液体稳态的重要调节因子。在炎症性肠病（IBD）患者的炎症肠道组织中，GN的基因表达和蛋白质水平受到抑制，但关于这些情况下鸟苷林血浆水平的了解却很少。我们旨在研究克罗恩病（CD）患者中前激素proGN和proUGN的空腹血浆水平，并将其与其他腹泻疾病患者以及肠道习惯正常者的水平进行比较。使用酶联免疫吸附测定（ELISA）分析了CD患者、家族性腹泻病（FGDS）患者、腹泻型肠易激综合征（IBS - D）患者和健康对照（HC）的血浆。与HC相比，CD和FGDS患者的空腹血浆前鸟苷林水平显著降低。在CD患者中，血浆proGN水平与哈维·布拉德肖指数以及24小时排便次数呈负相关。我们的数据表明，腹泻可能是血浆中proGN水平的一个决定因素，应在不同腹泻疾病的研究中进一步探索。
