California State University San Marcos, 333 S. Twin Oaks Valley Rd., San Marcos, CA, 92096, USA.
California State University San Marcos, 333 S. Twin Oaks Valley Rd., San Marcos, CA, 92096, USA.
Harmful Algae. 2020 Mar;93:101767. doi: 10.1016/j.hal.2020.101767. Epub 2020 Mar 4.
Reports of anatoxins poisoning of wildlife and domestic animals by toxigenic cyanobacteria in streams and rivers are increasing globally. Little is known about the taxonomy, morphology and genomics of anatoxins producing species, limiting our knowledge about their environmental preferences. We isolated three benthic non-heterocystous filamentous cyanobacterial strains from the Russian River in Northern California (USA), which produce anatoxin-a and dihydroanatoxin-a. Both 16S rRNA and protein sequence phylogenetic analyses showed that the strains represent a distinct new member of the cyanobacterial genus Microcoleus (Oscillatoriales). A novel species, Microcoleus anatoxicus is described and accompanied with light microscope photomicrographs, toxin profiles and the complete anatoxin-a gene cassette with the first description of the anaK gene in Microcoleus.
全球范围内，有关溪流和河流中产毒蓝藻引起野生动物和家畜类动物类毒素中毒的报告越来越多。对于产生类毒素的物种的分类学、形态学和基因组学知之甚少，这限制了我们对其环境偏好的了解。我们从美国加利福尼亚州北部的俄罗斯河（Russian River）中分离出了三种底栖非异形胞丝状蓝藻菌株，这些菌株可产生anatoxin-a 和 dihydroanatoxin-a。16S rRNA 和蛋白序列系统发育分析表明，这些菌株代表了蓝藻属 Microcoleus（Oscillatoriales）的一个新成员。描述了一个新物种，即 Microcoleus anatoxicus，并附有光镜照片、毒素图谱以及完整的 anatoxin-a 基因盒，其中首次在 Microcoleus 中描述了 anaK 基因。
