药物体外对牛视网膜色素上皮细胞溶酶体酶活性的影响。
Effect of drugs in vitro on lysosomal enzyme activities in bovine retinal pigment epithelial cells.
作者信息
Hayasaka S, Noda S, Setogawa T
机构信息
Department of Ophthalmology, Shimane Medical University, Izumo, Japan.
出版信息
Jpn J Ophthalmol. 1988;32(3):316-21.
We examined in vitro the effect of several drugs on the activities of lysosomal enzymes in the crude extract of bovine retinal pigment epithelial cells. Acid phosphatase, beta-D-glucuronidase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase, and alpha-D-mannosidase were used as lysosomal enzymes. Sodium iodate at 10(-6), 10(-5) and 10(-4) M, and potassium iodate at 10(-5) and 10(-4) M inhibited acid phosphatase activity. Ferrous chloride at 10(-5) and 10(-4) M suppressed beta-D-glucuronidase activity. Ferric chloride, indomethacin, chloroquine, chlorpromazine, 5-fluorouracil, tobramycin, daunomycin, oxalate and epinephrine had no or only minimal inhibitory effects on the lysosomal enzyme activities examined.
我们在体外研究了几种药物对牛视网膜色素上皮细胞粗提物中溶酶体酶活性的影响。酸性磷酸酶、β-D-葡萄糖醛酸酶、N-乙酰-β-D-氨基葡萄糖苷酶、α-L-岩藻糖苷酶和α-D-甘露糖苷酶被用作溶酶体酶。10^(-6)、10^(-5)和10^(-4)M的碘酸钠以及10^(-5)和10^(-4)M的碘酸钾抑制了酸性磷酸酶活性。10^(-5)和10^(-4)M的氯化亚铁抑制了β-D-葡萄糖醛酸酶活性。氯化铁、吲哚美辛、氯喹、氯丙嗪、5-氟尿嘧啶、妥布霉素、柔红霉素、草酸盐和肾上腺素对所检测的溶酶体酶活性没有或只有极小的抑制作用。
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