Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences.
Department of Anatomy and Functional Restorations, Division of Oral Health Sciences, Hiroshima University Graduate School of Biomedical Sciences.
Biomed Res. 2020;41(2):91-99. doi: 10.2220/biomedres.41.91.
The present study aimed to investigate the effect of a compressive force (CF) on the expression of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), and vascular endothelial growth factor (VEGF) in murine osteocytes (MLO-Y4) as well as animal study. After application of a CF for 1, 3, 6, and 12 h, gene and protein expression of RANKL, OPG, and VEGF in MLO-Y4 cells were determined by real-time PCR and enzyme-linked immunosorbent assay (ELISA). Furthermore, the effect of a stretch-activated (S-A) channel was examined by gadolinium (Gd) administration. In an animal experiment, the expression of these factors in osteocytes of alveolar bone was examined after experimental tooth movement in rats. After CF application, significant increases in RANKL, VEGF and RANKL/OPG ratio were shown. The upregulated gene and protein levels of these factors were reduced by Gd administration. After tooth movement, upregulated RANKL and VEGF were imunohistochemically shown in osteocytes of alveolar bone. These findings suggest that CF application on osteocytes elevates expression of osteoclast-inducing factor and angiogenesis factor in vivo and vitro.
本研究旨在探讨压缩力（CF）对鼠骨细胞（MLO-Y4）中核因子κB 受体激活剂配体（RANKL）、骨保护素（OPG）和血管内皮生长因子（VEGF）表达的影响，并进行动物研究。施加 CF 1、3、6 和 12 h 后，通过实时 PCR 和酶联免疫吸附试验（ELISA）测定 MLO-Y4 细胞中 RANKL、OPG 和 VEGF 的基因和蛋白表达。此外，通过钆（Gd）给药来检测伸展激活（S-A）通道的作用。在动物实验中，在大鼠实验性牙齿移动后，检查牙槽骨骨细胞中这些因子的表达。施加 CF 后，RANKL、VEGF 和 RANKL/OPG 比值显著增加。Gd 给药可降低这些因子的上调基因和蛋白水平。牙齿移动后，牙槽骨骨细胞中 RANKL 和 VEGF 的免疫组织化学显示上调。这些发现表明，CF 对骨细胞的应用可在体内和体外升高破骨细胞诱导因子和血管生成因子的表达。
