Abuohashish Hatem, Alamri Abdulaziz, Shahin Suliman, Almazrou Dalal, Alkhamis Taleb, Omar Omar
Department of Biomedical Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia.
Department of Preventive Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, 31441, Saudi Arabia.
Heliyon. 2023 May 12;9(5):e16217. doi: 10.1016/j.heliyon.2023.e16217. eCollection 2023 May.
This study aimed to evaluate the impact of bevacizumab on orthodontic tooth movement (OTM) in Wistar rats.
The OTM model was constructed by placing an orthodontic coil spring between the maxillary first molar and anterior tooth. Bevacizumab (Avastin®; 10 mg/kg twice per week) was started one week before the OTM and continued for 3 weeks. After 1 and 2 weeks, OTM distance and anterior tooth mobility were measured. Thereafter, the maxilla was dissected for micro-CT microarchitectural analysis, followed by histological analysis, and tartrate-resistant acid phosphatase (TRAP) staining. Moreover, the distributions of collagen fibers type-I and -III (Col-I and Col-III) were evaluated using Picro-Sirius red staining.
Orthodontic force prompted bone resorption and formation on the pressure and tension sides, respectively. Bevacizumab therapy resulted in a 42% increase of OTM, particularly after 2 weeks. Furthermore, bevacizumab disturbed the morphometric structure at both pressure and tension sites. The histological evaluation indicated about 35-44% fewer osteoblasts in the bevacizumab group, especially at the tension side, whereas the proportion of TRAP-positive osteoclasts at the pressure side was 34-37% higher than the control. The mature Col-I was reduced at the tension site by 33%, whereas the Col-III/Col-I ratio was enhanced by 20-44% at pressure and tension sites, after 2 weeks, in the bevacizumab group.
Anti-vascular bevacizumab therapy accentuates OTM in rat model, possibly through the enhancement of bone resorption, at the pressure side, and the reduction of bone formation, at the tension side as well as dysregulation of collagen fibers distribution.
本研究旨在评估贝伐单抗对Wistar大鼠正畸牙齿移动（OTM）的影响。
通过在上颌第一磨牙和前牙之间放置正畸螺旋弹簧构建OTM模型。在OTM开始前一周开始使用贝伐单抗（阿瓦斯汀®；10mg/kg，每周两次），持续3周。在1周和2周后，测量OTM距离和前牙移动度。此后，解剖上颌进行显微CT微观结构分析，随后进行组织学分析和抗酒石酸酸性磷酸酶（TRAP）染色。此外，使用天狼星红苦味酸染色评估I型和III型胶原纤维（Col-I和Col-III）的分布。
正畸力分别促使压力侧和张力侧的骨吸收和形成。贝伐单抗治疗使OTM增加了42%，尤其是在2周后。此外，贝伐单抗扰乱了压力侧和张力侧的形态结构。组织学评估表明，贝伐单抗组的成骨细胞减少了约35-44%，尤其是在张力侧，而压力侧TRAP阳性破骨细胞的比例比对照组高34-37%。在贝伐单抗组中，2周后张力侧成熟Col-I减少了33%，而压力侧和张力侧的Col-III/Col-I比值增加了20-44%。
抗血管生成的贝伐单抗治疗在大鼠模型中可促进OTM，可能是通过增强压力侧的骨吸收、减少张力侧的骨形成以及胶原纤维分布失调来实现的。
