Khaghanzadeh Narges, Naderi Nadereh, Pournasrollah Nazanin, Farahbakhsh Elahe, Kheirandish Masoumeh, Samiei Afshin
Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Diabetes Metab Syndr Obes. 2020 Apr 3;13:1015-1021. doi: 10.2147/DMSO.S238942. eCollection 2020.
Type 2 diabetes mellitus (T2DM) is a disease with a steadily increasing incidence throughout the world. Some molecules regulating the innate immune responses such as toll-like receptor 4 (TLR4) have shown to be involved in late diabetic complications. This study aimed to investigate the association of gene polymorphisms with clinicopathological aspects of T2DM in the Iranian population.
Two 896A>G and 1196C>T polymorphisms were assessed in 100 T2DM patients and 100 healthy controls using sequence-specific primers PCR. Demographic, anthropometric, and biochemical parameters were obtained from the participants.
After logistic regression, in 1196C>T, a significant association was shown between diabetic nephropathy (DN) and CT genotype (P= 0.04, OR= 4.35, CI= (1.04-18.1)). TG level has increased significantly in both T2DM and control subjects with CT genotype (P= 0.027, OR= 1.005, 95% CI= (1.001-1.01)). For 896A>G variant, a significant association was also detected between AG genotype and increased oral glucose tolerance test (OGTT) level (P= 0.048, OR= 1.003, 95% CI= (1.00-1.005)).
Although minor alleles of 1196C>T and 896A>G variants have not directly been associated with type 2 diabetes, by involving in the dysregulation of serum TG and blood sugar levels, they might increase the risk of DN.
2型糖尿病（T2DM）在全球的发病率呈稳步上升趋势。一些调节先天性免疫反应的分子，如Toll样受体4（TLR4），已被证明与糖尿病晚期并发症有关。本研究旨在探讨伊朗人群中基因多态性与T2DM临床病理特征之间的关联。
采用序列特异性引物PCR技术，对100例T2DM患者和100例健康对照者的两种896A>G和1196C>T多态性进行评估。从参与者那里获取人口统计学、人体测量学和生化参数。
经过逻辑回归分析，在1196C>T中，糖尿病肾病（DN）与CT基因型之间存在显著关联（P = 0.04，OR = 4.35，CI = (1.04 - 18.1)）。CT基因型的T2DM患者和对照者的甘油三酯（TG）水平均显著升高（P = 0.027，OR = 1.005，95% CI = (1.001 - 1.01)）。对于896A>G变异体，AG基因型与口服葡萄糖耐量试验（OGTT）水平升高之间也存在显著关联（P = 0.048，OR = 1.003，95% CI = (1.00 - 1.005)）。
虽然1196C>T和896A>G变异体的次要等位基因与2型糖尿病没有直接关联，但通过参与血清TG和血糖水平的失调，它们可能会增加DN的风险。
