Matsubara J, Kamiyama T, Miyoshi T, Ueda H, Saito M, Nakagawa M
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Lab Anim. 1988 Oct;22(4):304-8. doi: 10.1258/002367788780746214.
Guineapig antibodies to Streptococcus pneumoniae (SPN) serotype 19F were detected by an enzyme-linked immunosorbent assay using a simple procedure. In experimentally infected hosts, antibody was detectable as early as 2 to 3 weeks after infection, and high titres were maintained for a long period. Antibodies higher than 1:64 were regarded as specific. In a field study, high antibody titres were shown in SPN enzootic colonies in contrast to negative or low antibody titres in a majority of the animals from non-enzootic and SPF colonies.
采用一种简单的程序，通过酶联免疫吸附测定法检测了豚鼠针对肺炎链球菌（SPN）19F血清型的抗体。在实验感染的宿主中，感染后最早2至3周即可检测到抗体，并且高滴度可长期维持。高于1:64的抗体被视为特异性抗体。在一项实地研究中，与来自非疫区和无特定病原体（SPF）群体的大多数动物的阴性或低抗体滴度形成对比的是，在SPN疫区群体中显示出高抗体滴度。
