一种用于监测豚鼠和兔子支气管败血波氏杆菌抗体的酶联免疫吸附测定（ELISA）。

An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies.

作者信息

Boot R, Bakker R H, Thuis H, Veenema J L

机构信息

Department of Laboratory Animal Microbiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.

出版信息

Lab Anim. 1993 Oct;27(4):342-9. doi: 10.1258/002367793780745624.

DOI:10.1258/002367793780745624

PMID:8277707

Abstract

An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies specific to Bordetella bronchiseptica in guineapigs and rabbits was developed. In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. The ELISA showed a sensitivity of 100% and a specificity of 90% in guineapigs. In rabbits the sensitivity and specificity of the ELISA were 97% and 91% respectively. In rabbit sera from infected colonies, ELISA activity showed a statistically significant correlation with titres obtained in the micro-agglutination test. Since serologically unrelated strains of the bacterium exist, the monitoring of animals for B. bronchiseptica infection by ELISA should be performed with various antigens.

摘要

开发了一种用于监测豚鼠和兔体内支气管败血波氏杆菌特异性抗体的酶联免疫吸附测定（ELISA）。在豚鼠和兔的常规及无特定病原体（SPF）群体中，与从呼吸道进行选择性培养相比，ELISA在检测感染动物方面同样成功。ELISA在豚鼠中的敏感性为100%，特异性为90%。在兔中，ELISA的敏感性和特异性分别为97%和91%。在来自感染群体的兔血清中，ELISA活性与微量凝集试验获得的滴度呈统计学显著相关。由于存在血清学不相关的该细菌菌株，通过ELISA监测动物的支气管败血波氏杆菌感染应使用多种抗原进行。