四环素和地西泮对分离的大鼠胰岛胰岛素分泌及腺苷酸环化酶活性的影响。

Effects of tetracycline and diazepam on insulin secretion and adenylyl cyclase activity of isolated rat islets of Langerhans.

作者信息

El-Denshary E S, Ismail N A, Montague W

出版信息

Diabete Metab. 1977 Mar;3(1):3-6.

Abstract

Tetracycline (2 and 20 micronM) inhibited glucose-stimulated insulin secretion from isolated rat islets of Langerhans, although it failed to alter tolbutamide-stimulated insulin release. Diazepam (10 micronM) potentiated tolbutamide-stimulated insulin release and at a concentration of 1 mM increased glucose-stimulated insulin release. Tetracycline (20 and 200 micronM) or diazepam (10, 100 and 1,000 micronM) inhibited adenylyl cyclase activity of islets homogenate. These results suggest that the effect of tetracycline on insulin secretion might be in part due to inhibition of adenylyl cyclase of the islets. However, the effect of diazepam on insulin secretion is not mediated through the adenylyl cyclase system.

摘要

四环素（2和20微摩尔）抑制了从分离的大鼠胰岛中葡萄糖刺激的胰岛素分泌，尽管它未能改变甲苯磺丁脲刺激的胰岛素释放。地西泮（10微摩尔）增强了甲苯磺丁脲刺激的胰岛素释放，并且在1毫摩尔的浓度下增加了葡萄糖刺激的胰岛素释放。四环素（20和200微摩尔）或地西泮（10、100和1000微摩尔）抑制了胰岛匀浆的腺苷酸环化酶活性。这些结果表明，四环素对胰岛素分泌的影响可能部分归因于对胰岛腺苷酸环化酶的抑制。然而，地西泮对胰岛素分泌的影响不是通过腺苷酸环化酶系统介导的。