Khosravani Marjan, Soltan Dallal Mohammad Mehdi, Norouzi Mehdi
Department of Microbiology, Fars Science and Research Branch, Islamic Azad University, Shiraz, Iran.
Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran.
Iran J Public Health. 2020 Jan;49(1):134-144.
The AcrB efflux pump in Salmonella species plays a significant role in the development of antibiotic resistance in ciprofloxacin-resistant . This study aimed to investigate the anti-efflux pump activity of extracts among strains.
The hydroalcoholic, aqueous, and hexanolic extracts of were prepared and phytochemical composition of extract was determined using gas chromatography/mass spectrometry (GC/MS) method. After antibiogram, the AcrB efflux pump was detected in ciprofloxacin intermediate and resistant strains using cartwheel and Polymerase chain reaction (PCR) methods. Finally, minimum inhibitory concentrations (MIC) of extracts against strains were evaluated. After treatment of strains with sub-MIC concentrations of extracts, the expression level of AcrB efflux pump gene was evaluated using Real-Time PCR.
Phytochemical analysis of extracts using GC/MS method showed that hexadecanoic acid, ethyl ester (30.7%), and cyclopropane,1-(1-hydroxy-1-heptyl)-2-methylene-3-pentyl (17.8%) were the most dominant volatile components volatile compounds in the extract. The results of antibiogram, cartwheel and PCR methods showed that among 20 strains of that were resistant and intermediate to ciprofloxacin, 16 strains had AcrB efflux pumps. Finally, Real-Time PCR results showed a significant down-regulation of gene in strains.
A. had anti-efflux activity and this plant can potentially be used as a natural efflux inhibitor for strains.
沙门氏菌中的AcrB外排泵在耐环丙沙星菌株的抗生素耐药性发展中起重要作用。本研究旨在调查[植物名称]提取物对[细菌名称]菌株的抗外排泵活性。
制备了[植物名称]的水醇提取物、水提取物和己烷提取物，并采用气相色谱/质谱（GC/MS）法测定提取物的植物化学成分。在进行药敏试验后，使用车轮试验和聚合酶链反应（PCR）方法在环丙沙星中介和耐药的[细菌名称]菌株中检测AcrB外排泵。最后，评估提取物对[细菌名称]菌株的最低抑菌浓度（MIC）。在用亚MIC浓度的提取物处理[细菌名称]菌株后，使用实时PCR评估AcrB外排泵基因的表达水平。
采用GC/MS法对提取物进行植物化学分析表明，十六烷酸乙酯（30.7%）和环丙烷，1-（1-羟基-1-庚基）-2-亚甲基-3-戊基（17.8%）是提取物中最主要的挥发性成分。药敏试验、车轮试验和PCR方法的结果表明，在20株对环丙沙星耐药和中介的[细菌名称]菌株中, 16株具有AcrB外排泵。最后，实时PCR结果显示[细菌名称]菌株中[基因名称]基因显著下调。
[植物名称]具有抗外排活性，这种植物有可能用作[细菌名称]菌株的天然外排抑制剂。
